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- W1986017237 abstract "The β-galactosidases (β-Gals) of Lactobacillus reuteri L103 and L461 proved to be suitable biocatalysts for the production of prebiotic galacto-oligosaccharides (GOS) from lactose. Maximum GOS yields were 38% when using an initial lactose concentration of 205 g/L and at ∼80% lactose conversion. The product mixtures were analyzed by capillary electrophoresis (CE) and high-performance anion-exchange chromatography with pulsed amperometric detection (HPAEC-PAD). Disaccharides other than lactose and trisaccharides made up the vast majority of GOS formed. The main products were identified as β-d-Galp-(1→6)-d-Glc (allolactose), β-d-Galp-(1→6)-d-Gal, β-d-Galp-(1→3)-d-Gal, β-d-Galp-(1→6)-Lac, and β-d-Galp-(1→3)-Lac. There were no major products with β1→4 linkages formed. Both intermolecular and intramolecular transgalactosylation were observed. d-Galactose proved to be a very efficient galactosyl acceptor; thus, a relatively large amount of galactobioses was formed. Monosaccharides could be conveniently separated from the mixture by chromatography using a strong cation-exchange resin. Keywords: Lactobacillus; β-galactosidase; galacto-oligosaccharides; prebiotics; transgalactosylation" @default.
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- W1986017237 date "2006-06-21" @default.
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- W1986017237 title "Production of Prebiotic Galacto-Oligosaccharides from Lactose Using β-Galactosidases from <i>Lactobacillus reuteri</i>" @default.
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- W1986017237 doi "https://doi.org/10.1021/jf053127m" @default.
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