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• W1986042279 endingPage "111607" @default.
• W1986042279 startingPage "111607" @default.
• W1986042279 abstract "The spectroscopy of analyte-specific molecular vibrations in tissue thin sections has opened up a path toward histopathology without the need for tissue staining. However, biomedical vibrational imaging has not yet advanced from academic research to routine histopathology due to long acquisition times for the microscopic hyperspectral images and/or cost and availability of the necessary equipment. Here we show that the combination of a fast-tuning quantum cascade laser with a microbolometer array detector allows for a rapid image acquisition and bares the potential for substantial cost reduction. A 3.1×2.8 mm2 unstained thin section of mouse jejunum has been imaged in the 9.2 to 9.7 μm wavelength range (spectral resolution ∼1 cm−1) within 5 min with diffraction limited spatial resolution. The comparison of this hyperspectral imaging approach with standard Fourier transform infrared imaging or mapping of the identical sample shows a reduction in acquisition time per wavenumber interval and image area by more than one or three orders of magnitude, respectively." @default.
• W1986042279 created "2016-06-24" @default.
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• W1986042279 date "2014-06-26" @default.
• W1986042279 modified "2023-09-27" @default.
• W1986042279 title "Quantum cascade laser–based hyperspectral imaging of biological tissue" @default.
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• W1986042279 doi "https://doi.org/10.1117/1.jbo.19.11.111607" @default.
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