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• W1986078634 abstract "By loading cells in culture with acetylcholine (ACh) we have characterized a calcium-dependent release mechanism and shown that it was expressed independently of synthesis or storage of ACh. (Israël et al., 1994, Neurochemistry International 37, 1475–1483; Falk-Vairant et al., 1996a, Proc. Natl. Acad. Sci. U.S.A. 93, 5203–5207; Falk-Vairant et al., 1996b, Neuroscience 75, 353–360; Falk-Vairant et al., 1996c, Journal of Neuroscience Research 45, 195–201). The transmitter loading procedure was applied to two other transmitters, γ-aminobutyric acid (GABA) and glutamate (Glu). We could then study the specificity of the release mechanism for the three transmitters in a variety of cell lines, including neural-derived cells. Four different calcium-dependent release phenotypes were identified: two were specific for ACh or GABA, and two coreleased two transmitters ACh and GABA but not Glu, or ACh and Glu but not GABA. We conclude that release mechanisms having different specificities are expressed by the cell lines studied, they become functional after loading the cells with the relevant transmitters. These observations will help the identification of proteins controlling the specificity of release, and provide an interesting model for pharmacological studies." @default.
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• W1986078634 date "1997-11-01" @default.
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• W1986078634 title "Calcium-dependent release specificities of various cell lines loaded with different transmitters" @default.
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• W1986078634 doi "https://doi.org/10.1016/s0028-3908(97)00149-4" @default.
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