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- W1986099961 abstract "Summary The development of fluorochrome‐conjugated peptide–major histocompatibility complex (pMHC) multimers in conjunction with continuing advances in flow cytometry has transformed the study of antigen‐specific T cells by enabling their visualization, enumeration, phenotypic characterization and isolation from ex vivo samples. Here, we bring together and discuss some of the ‘tricks’ that can be used to get the most out of pMHC multimers. These include: (1) simple procedures that can substantially enhance the staining intensity of cognate T cells with pMHC multimers; (2) the use of pMHC multimers to stain T cells with very‐low‐affinity T‐cell receptor (TCR)/pMHC interactions, such as those that typically predominate in tumour‐specific responses; and (3) the physical grading and clonotypic dissection of antigen‐specific T cells based on the affinity of their cognate TCR using mutant pMHC multimers in conjunction with new approaches to the molecular analysis of TCR gene expression. We also examine how soluble pMHC can be used to examine T‐cell activation, manipulate T‐cell responses and study allogeneic and superantigen interactions with TCRs. Finally, we discuss the problems that arise with pMHC class II (pMHCII) multimers because of the low affinity of TCR/pMHCII interactions and lack of ‘coreceptor help’." @default.
- W1986099961 created "2016-06-24" @default.
- W1986099961 creator A5000491178 @default.
- W1986099961 creator A5008376558 @default.
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- W1986099961 creator A5024394000 @default.
- W1986099961 creator A5046316209 @default.
- W1986099961 creator A5058182638 @default.
- W1986099961 date "2009-01-05" @default.
- W1986099961 modified "2023-10-16" @default.
- W1986099961 title "Tricks with tetramers: how to get the most from multimeric peptide-MHC" @default.
- W1986099961 cites W1479760379 @default.
- W1986099961 cites W1488545150 @default.
- W1986099961 cites W1507930766 @default.
- W1986099961 cites W1519418301 @default.
- W1986099961 cites W1534025594 @default.
- W1986099961 cites W1535034989 @default.
- W1986099961 cites W1544285519 @default.
- W1986099961 cites W1568150013 @default.
- W1986099961 cites W1569766809 @default.
- W1986099961 cites W1589097025 @default.
- W1986099961 cites W1594353637 @default.
- W1986099961 cites W1600259832 @default.
- W1986099961 cites W1602273859 @default.
- W1986099961 cites W1612041908 @default.
- W1986099961 cites W1643293176 @default.
- W1986099961 cites W1677807424 @default.
- W1986099961 cites W1931699789 @default.
- W1986099961 cites W1963569988 @default.
- W1986099961 cites W1964013889 @default.
- W1986099961 cites W1964115378 @default.
- W1986099961 cites W1966989202 @default.
- W1986099961 cites W1967335584 @default.
- W1986099961 cites W1967684158 @default.
- W1986099961 cites W1968112727 @default.
- W1986099961 cites W1968611188 @default.
- W1986099961 cites W1975724971 @default.
- W1986099961 cites W1976063319 @default.
- W1986099961 cites W1978094859 @default.
- W1986099961 cites W1979773132 @default.
- W1986099961 cites W1986248194 @default.
- W1986099961 cites W1990328636 @default.
- W1986099961 cites W1990694172 @default.
- W1986099961 cites W1994311739 @default.
- W1986099961 cites W2003324302 @default.
- W1986099961 cites W2006593948 @default.
- W1986099961 cites W2008284182 @default.
- W1986099961 cites W2011110777 @default.
- W1986099961 cites W2011746398 @default.
- W1986099961 cites W2012108097 @default.
- W1986099961 cites W2015569874 @default.
- W1986099961 cites W2015866042 @default.
- W1986099961 cites W2016918975 @default.
- W1986099961 cites W2022924308 @default.
- W1986099961 cites W2026875548 @default.
- W1986099961 cites W2029607309 @default.
- W1986099961 cites W2040606992 @default.
- W1986099961 cites W2042973385 @default.
- W1986099961 cites W2045119189 @default.
- W1986099961 cites W2053365848 @default.
- W1986099961 cites W2053740579 @default.
- W1986099961 cites W2055184978 @default.
- W1986099961 cites W2056069176 @default.
- W1986099961 cites W2060165194 @default.
- W1986099961 cites W2060354653 @default.
- W1986099961 cites W2061285041 @default.
- W1986099961 cites W2063986770 @default.
- W1986099961 cites W2065430316 @default.
- W1986099961 cites W2067069532 @default.
- W1986099961 cites W2067353129 @default.
- W1986099961 cites W2069126031 @default.
- W1986099961 cites W2069717701 @default.
- W1986099961 cites W2070530924 @default.
- W1986099961 cites W2070576765 @default.
- W1986099961 cites W2071423255 @default.
- W1986099961 cites W2072723772 @default.
- W1986099961 cites W2079355826 @default.
- W1986099961 cites W2080086146 @default.
- W1986099961 cites W2080212882 @default.
- W1986099961 cites W2080482588 @default.
- W1986099961 cites W2081533843 @default.
- W1986099961 cites W2082794963 @default.
- W1986099961 cites W2083331853 @default.
- W1986099961 cites W2084870747 @default.
- W1986099961 cites W2086336800 @default.
- W1986099961 cites W2087121403 @default.
- W1986099961 cites W2090300527 @default.
- W1986099961 cites W2094213517 @default.
- W1986099961 cites W2094676264 @default.
- W1986099961 cites W2096634178 @default.
- W1986099961 cites W2106160458 @default.
- W1986099961 cites W2108861854 @default.
- W1986099961 cites W2109584015 @default.
- W1986099961 cites W2110710427 @default.
- W1986099961 cites W2111359040 @default.
- W1986099961 cites W2112270368 @default.
- W1986099961 cites W2112505356 @default.
- W1986099961 cites W2112859782 @default.