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- W1986116502 abstract "A sensitive bright field/fluorescent histochemical staining method has been developed that reveals endogenous aluminum in subcellular structures. The method, achievable within 30 min, is based on phloxine B and phosphotungstic acid, with ethanol differentiation. Hematoxylin is used for nuclear and fast green FCF for cytoplasmic counterstaining. To test the method's specificity, we incubated living neuroblastoma cells overnight in culture media containing aluminum, calcium, iron, copper or zinc, or no added metal ions. After fixing the cells and applying the staining method, only cultures exposed to aluminum stained magenta. Applying the method to paraffin embedded tissue sections pretreated with one of two chelating agents that remove aluminum demonstrated less magenta staining in the chelated sections than in adjacent unchelated sections. Immersing sections overnight in solutions containing exogenous aluminum had no observable effect on staining for endogenous aluminum; therefore, it is unlikely that any exogenous aluminum present in histological reagents would alter the method's staining results." @default.
- W1986116502 created "2016-06-24" @default.
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- W1986116502 date "2004-10-01" @default.
- W1986116502 modified "2023-10-15" @default.
- W1986116502 title "A bright field/fluorescent stain for aluminum: its specificity, validation, and staining characteristics" @default.
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- W1986116502 doi "https://doi.org/10.1080/10520290400015423" @default.
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