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- W1986144871 abstract "Agrobacterium tumefaciens transfers DNA to plant cells as a single-stranded DNA molecule (the T-strand) covalently linked to VirD2 protein. VirD2 contains nuclear localization signal sequences that presumably help direct the T-strand to the plant nucleus. We identified a tomato cDNA clone, DIG3, that encodes a protein that interacts with the C-terminal region of VirD2. DIG3 encodes an enzymatically active type 2C serine/threonine protein phosphatase. Overexpression of DIG3 in tobacco BY-2 protoplasts inhibited nuclear import of a beta-glucuronidase-VirD2 nuclear localization signal fusion protein. Thus, DIG3 may be involved in nuclear import of the VirD2 protein and, consequently, the VirD2/transferred DNA complex." @default.
- W1986144871 created "2016-06-24" @default.
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- W1986144871 date "2004-03-26" @default.
- W1986144871 modified "2023-09-27" @default.
- W1986144871 title "Expression of plant protein phosphatase 2C interferes with nuclear import of the <i>Agrobacterium</i> T-complex protein VirD2" @default.
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- W1986144871 doi "https://doi.org/10.1073/pnas.0300084101" @default.
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