Matches in SemOpenAlex for { <https://semopenalex.org/work/W1986167910> ?p ?o ?g. }
- W1986167910 abstract "Methods for specific immobilization, isolation and labeling of proteins are central to the elucidation of cellular functions. Based on bacterial repressor proteins, which bind to specific target sequences in response to small molecules (macrolide and tetracycline antibiotics) or environmental parameters (temperature), we have developed a set of protein tags (RepTAGs), which enable reversible immobilization of the protein of interest on a solid support for the isolation and quantification as well as for the specific labeling of target proteins with fluorescent dyes for tracking them within a complex protein mixture. Similarly, live mammalian cells were specifically labeled with a fluorescent operator sequence bound to RepTAGs, which were directed towards the cell surface for easy discrimination between transfected and untransfected cell populations. Based on the drug-responsive RepTAG-DNA interactions, it was also possible to quantify or discover antibiotics in environmental samples or compound libraries by means of rapid, sensitive detection methods involving fluorescence polarization and bioluminescence. We believe that the universally applicable RepTAGs will become essential for the analysis and manipulation of proteins in the most diverse areas of protein chemistry and cell biology." @default.
- W1986167910 created "2016-06-24" @default.
- W1986167910 creator A5008483564 @default.
- W1986167910 creator A5077615505 @default.
- W1986167910 creator A5078274825 @default.
- W1986167910 creator A5082252506 @default.
- W1986167910 creator A5084630304 @default.
- W1986167910 date "2007-12-15" @default.
- W1986167910 modified "2023-09-26" @default.
- W1986167910 title "RepTAGs: Universal tags for isolation and labeling of proteins, for labeling live mammalian cells and for drug discovery" @default.
- W1986167910 cites W1645799104 @default.
- W1986167910 cites W1930402573 @default.
- W1986167910 cites W1942606329 @default.
- W1986167910 cites W1966291152 @default.
- W1986167910 cites W1972892361 @default.
- W1986167910 cites W1974068980 @default.
- W1986167910 cites W1980785006 @default.
- W1986167910 cites W1983940758 @default.
- W1986167910 cites W1996022620 @default.
- W1986167910 cites W1998963787 @default.
- W1986167910 cites W2030319745 @default.
- W1986167910 cites W2032123987 @default.
- W1986167910 cites W2032619610 @default.
- W1986167910 cites W2035640095 @default.
- W1986167910 cites W2035713613 @default.
- W1986167910 cites W2036076986 @default.
- W1986167910 cites W2036719828 @default.
- W1986167910 cites W2040516865 @default.
- W1986167910 cites W2043260079 @default.
- W1986167910 cites W2044639011 @default.
- W1986167910 cites W2049862836 @default.
- W1986167910 cites W2066706173 @default.
- W1986167910 cites W2074998346 @default.
- W1986167910 cites W2082520438 @default.
- W1986167910 cites W2090429649 @default.
- W1986167910 cites W2111929724 @default.
- W1986167910 cites W2113384803 @default.
- W1986167910 cites W2114522205 @default.
- W1986167910 cites W2123505432 @default.
- W1986167910 cites W2130840822 @default.
- W1986167910 cites W2135158743 @default.
- W1986167910 cites W2136646494 @default.
- W1986167910 cites W2154568825 @default.
- W1986167910 cites W2157668714 @default.
- W1986167910 cites W2171535756 @default.
- W1986167910 doi "https://doi.org/10.1002/bit.21485" @default.
- W1986167910 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17492694" @default.
- W1986167910 hasPublicationYear "2007" @default.
- W1986167910 type Work @default.
- W1986167910 sameAs 1986167910 @default.
- W1986167910 citedByCount "0" @default.
- W1986167910 crossrefType "journal-article" @default.
- W1986167910 hasAuthorship W1986167910A5008483564 @default.
- W1986167910 hasAuthorship W1986167910A5077615505 @default.
- W1986167910 hasAuthorship W1986167910A5078274825 @default.
- W1986167910 hasAuthorship W1986167910A5082252506 @default.
- W1986167910 hasAuthorship W1986167910A5084630304 @default.
- W1986167910 hasConcept C104317684 @default.
- W1986167910 hasConcept C121332964 @default.
- W1986167910 hasConcept C142613039 @default.
- W1986167910 hasConcept C150194340 @default.
- W1986167910 hasConcept C158448853 @default.
- W1986167910 hasConcept C171852809 @default.
- W1986167910 hasConcept C185592680 @default.
- W1986167910 hasConcept C2780923616 @default.
- W1986167910 hasConcept C501593827 @default.
- W1986167910 hasConcept C52909226 @default.
- W1986167910 hasConcept C552990157 @default.
- W1986167910 hasConcept C55493867 @default.
- W1986167910 hasConcept C62520636 @default.
- W1986167910 hasConcept C70721500 @default.
- W1986167910 hasConcept C74187038 @default.
- W1986167910 hasConcept C86803240 @default.
- W1986167910 hasConcept C91881484 @default.
- W1986167910 hasConcept C95444343 @default.
- W1986167910 hasConceptScore W1986167910C104317684 @default.
- W1986167910 hasConceptScore W1986167910C121332964 @default.
- W1986167910 hasConceptScore W1986167910C142613039 @default.
- W1986167910 hasConceptScore W1986167910C150194340 @default.
- W1986167910 hasConceptScore W1986167910C158448853 @default.
- W1986167910 hasConceptScore W1986167910C171852809 @default.
- W1986167910 hasConceptScore W1986167910C185592680 @default.
- W1986167910 hasConceptScore W1986167910C2780923616 @default.
- W1986167910 hasConceptScore W1986167910C501593827 @default.
- W1986167910 hasConceptScore W1986167910C52909226 @default.
- W1986167910 hasConceptScore W1986167910C552990157 @default.
- W1986167910 hasConceptScore W1986167910C55493867 @default.
- W1986167910 hasConceptScore W1986167910C62520636 @default.
- W1986167910 hasConceptScore W1986167910C70721500 @default.
- W1986167910 hasConceptScore W1986167910C74187038 @default.
- W1986167910 hasConceptScore W1986167910C86803240 @default.
- W1986167910 hasConceptScore W1986167910C91881484 @default.
- W1986167910 hasConceptScore W1986167910C95444343 @default.
- W1986167910 hasLocation W19861679101 @default.
- W1986167910 hasLocation W19861679102 @default.
- W1986167910 hasOpenAccess W1986167910 @default.
- W1986167910 hasPrimaryLocation W19861679101 @default.
- W1986167910 hasRelatedWork W1076866363 @default.
- W1986167910 hasRelatedWork W1973833245 @default.
- W1986167910 hasRelatedWork W1988606893 @default.