FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1986246254> ?p ?o ?g. }

• W1986246254 endingPage "331" @default.
• W1986246254 startingPage "324" @default.
• W1986246254 abstract "Two monoclonal antibodies, termed nnIE11 and nnIG11, were generated against the murine thymic stromal lymphopoietin receptor, mTSLPR, using traditional hybridoma technology. The antibody-producing hybridoma clones were obtained by fusing P3X63-Ag8.653 myeloma cells with splenocytes from Balb/c mice immunized with anti-FLAG M2 affinity-purified FLAG-tagged mTSLPR from pSVL-mTSLPR-FLAG-transfected COS cells and Ni-NTA-purified his-tagged mTSLPR from recombinant FastBacHisB-mdelta1 baculovirus-infected Sf9 cells. Several monoclonal anti-mTSLPR-specific hybridoma clones were obtained and two of these clones are further characterized here. The generated antibodies could in an immunoblotting identify baculovirus-expressed mTSLPR proteins with a molecular weight corresponding to 50 kDa. Both immunoblotting and ELISA with recombinant mouse TSLPR/Fc chimera as antigen, having only the N-terminal domain of mTSLPR present, indicated that the generated monoclonal antibodies identify the C-terminus of mTSLPR. Although sandwich ELISAs performed with a goat anti-mTSLPR antiserum as capture antibody and nnIE11 as indicator antibody were able to detect mTSLPR in the range of 5 ng/ml, no souble mTSLPR could be observed in serum samples from CBA/H, Balb/c and C57Bl/6 mice." @default.
• W1986246254 created "2016-06-24" @default.
• W1986246254 creator A5001977526 @default.
• W1986246254 creator A5019389291 @default.
• W1986246254 creator A5038123771 @default.
• W1986246254 creator A5039627599 @default.
• W1986246254 creator A5060571646 @default.
• W1986246254 creator A5065556827 @default.
• W1986246254 date "2005-05-01" @default.
• W1986246254 modified "2023-10-16" @default.
• W1986246254 title "Soluble thymic stromal lymphopoietin receptors are absent in murine sera - detection with anti-mTSLPR monoclonal antibodies" @default.
• W1986246254 cites W1505782089 @default.
• W1986246254 cites W1524000396 @default.
• W1986246254 cites W1558665965 @default.
• W1986246254 cites W1570212613 @default.
• W1986246254 cites W1971731547 @default.
• W1986246254 cites W1979026253 @default.
• W1986246254 cites W1982787810 @default.
• W1986246254 cites W1988567136 @default.
• W1986246254 cites W1993940396 @default.
• W1986246254 cites W2003967740 @default.
• W1986246254 cites W2021950135 @default.
• W1986246254 cites W2029606100 @default.
• W1986246254 cites W2032650588 @default.
• W1986246254 cites W2040888475 @default.
• W1986246254 cites W2049836784 @default.
• W1986246254 cites W2072622802 @default.
• W1986246254 cites W2087691985 @default.
• W1986246254 cites W2088167128 @default.
• W1986246254 cites W2100837269 @default.
• W1986246254 cites W2101108802 @default.
• W1986246254 cites W2120056767 @default.
• W1986246254 cites W2166667170 @default.
• W1986246254 cites W4234223354 @default.
• W1986246254 cites W859299 @default.
• W1986246254 doi "https://doi.org/10.1111/j.1600-0463.2005.apm_113503.x" @default.
• W1986246254 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16011658" @default.
• W1986246254 hasPublicationYear "2005" @default.
• W1986246254 type Work @default.
• W1986246254 sameAs 1986246254 @default.
• W1986246254 citedByCount "0" @default.
• W1986246254 crossrefType "journal-article" @default.
• W1986246254 hasAuthorship W1986246254A5001977526 @default.
• W1986246254 hasAuthorship W1986246254A5019389291 @default.
• W1986246254 hasAuthorship W1986246254A5038123771 @default.
• W1986246254 hasAuthorship W1986246254A5039627599 @default.
• W1986246254 hasAuthorship W1986246254A5060571646 @default.
• W1986246254 hasAuthorship W1986246254A5065556827 @default.
• W1986246254 hasConcept C103878061 @default.
• W1986246254 hasConcept C104317684 @default.
• W1986246254 hasConcept C153911025 @default.
• W1986246254 hasConcept C159047783 @default.
• W1986246254 hasConcept C159654299 @default.
• W1986246254 hasConcept C170493617 @default.
• W1986246254 hasConcept C189976730 @default.
• W1986246254 hasConcept C203014093 @default.
• W1986246254 hasConcept C2777773057 @default.
• W1986246254 hasConcept C2778070265 @default.
• W1986246254 hasConcept C2778502174 @default.
• W1986246254 hasConcept C40767141 @default.
• W1986246254 hasConcept C54009773 @default.
• W1986246254 hasConcept C542903549 @default.
• W1986246254 hasConcept C54355233 @default.
• W1986246254 hasConcept C55493867 @default.
• W1986246254 hasConcept C81885089 @default.
• W1986246254 hasConcept C86803240 @default.
• W1986246254 hasConcept C91523082 @default.
• W1986246254 hasConceptScore W1986246254C103878061 @default.
• W1986246254 hasConceptScore W1986246254C104317684 @default.
• W1986246254 hasConceptScore W1986246254C153911025 @default.
• W1986246254 hasConceptScore W1986246254C159047783 @default.
• W1986246254 hasConceptScore W1986246254C159654299 @default.
• W1986246254 hasConceptScore W1986246254C170493617 @default.
• W1986246254 hasConceptScore W1986246254C189976730 @default.
• W1986246254 hasConceptScore W1986246254C203014093 @default.
• W1986246254 hasConceptScore W1986246254C2777773057 @default.
• W1986246254 hasConceptScore W1986246254C2778070265 @default.
• W1986246254 hasConceptScore W1986246254C2778502174 @default.
• W1986246254 hasConceptScore W1986246254C40767141 @default.
• W1986246254 hasConceptScore W1986246254C54009773 @default.
• W1986246254 hasConceptScore W1986246254C542903549 @default.
• W1986246254 hasConceptScore W1986246254C54355233 @default.
• W1986246254 hasConceptScore W1986246254C55493867 @default.
• W1986246254 hasConceptScore W1986246254C81885089 @default.
• W1986246254 hasConceptScore W1986246254C86803240 @default.
• W1986246254 hasConceptScore W1986246254C91523082 @default.
• W1986246254 hasIssue "5" @default.
• W1986246254 hasLocation W19862462541 @default.
• W1986246254 hasLocation W19862462542 @default.
• W1986246254 hasOpenAccess W1986246254 @default.
• W1986246254 hasPrimaryLocation W19862462541 @default.
• W1986246254 hasRelatedWork W1973692620 @default.
• W1986246254 hasRelatedWork W1986246254 @default.
• W1986246254 hasRelatedWork W2345890204 @default.
• W1986246254 hasRelatedWork W2349557033 @default.
• W1986246254 hasRelatedWork W2353735430 @default.
• W1986246254 hasRelatedWork W2361347978 @default.
• W1986246254 hasRelatedWork W2373649475 @default.

• next