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- W1986249618 abstract "We have examined some of the properties of the DNA-agar binding technique, using mammalian DNA. Fragments of mammalian DNA incubated with homologous DNA trapped in agar gels form stable duplex structures, though under the more stringent conditions occurring in solution mammalian DNA at low concentration renatures little, if at all. This paradoxical situation may be resolved on the assumption that, in agar, relatively short lengths of duplex structure suffice to stabilize fragmented DNA, so that the number of potential binding sites is considerably greater, thus partly compensating for the greater molecular heterogeneity of mammalian compared to bacterial DNA. The proportion of mammalian DNA fragments bound in a DNA-agar gel is smaller than when the source of DNA is bacterial. Further, fragments which fail to bind on a first incubation also bind poorly on subsequent incubations. We show that these features are not due to any gross technical artifact, nor in the main to the formation of complexes between DNA fragments. The characteristic binding behaviour of mammalian as opposed to bacterial DNA may be attributed to the shorter average length of duplex formed in the mammalian situation, taken in conjunction with the persistence of intrastrand secondary structure in denatured DNA. Specificity of binding is lower for mammalian than for bacterial DNA, and the complexes formed have been experimentally shown to be less stable." @default.
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- W1986249618 date "1965-06-01" @default.
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- W1986249618 title "Specific duplex formation in vitro of mammalian DNA" @default.
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- W1986249618 doi "https://doi.org/10.1016/s0022-2836(65)80263-7" @default.
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