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- W1986285429 abstract "C3NeF has only been characterized as an IgG molecule which is present in the sera of patients with MPGN. It acts by stabilizing the alternative pathway C3/C5 convertase (C3bBb). When peripheral blood lymphocytes (PBL) from newborns, normal adults, or patients with MPGN are cultured in fetal calf serum with pokeweed mitogen, however, C3NeF is elaborated as both IgG and IgM molecules. Thus, culture supernatants (after adsorption with E, EC3b, B, P) were added to sheep erythrocytes bearing C3bBb (EC3bBb). The cells were then quantitated for bound human IgG or IgM by ELISA and decayed at 30° with measurement of residual convertase activity (RCA). Cells reacted with cultures from both normals and patients contained variable amounts of IgG (8-81 ng) and IgM (5-22 ng); decay curves showed a clear decrease in slope over control. IgM and IgG isolated from the culture supernatants by NH4SO4 precepitation and Protein A adsorption were both active as C3NeF. When IgM and IgG C3NeF from normal or MPGN cultures were mixed, there was a substantial decrease (40-56%) in the deposition of both molecules. RCA was unchanged with the mixture of normal Ig's. In the mixture from MPGN cultures, however, there was a marked decrease in stabilization with a shift of decay curves toward normal. These results indicate that IgM C3NeF may inhibit IgG C3NeF and, therefore, be important in the control of complement activity in MPGN." @default.
- W1986285429 created "2016-06-24" @default.
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- W1986285429 date "1985-04-01" @default.
- W1986285429 modified "2023-10-14" @default.
- W1986285429 title "1637 DEMONSTRATION AND MECHANISM OF ACTION OF IgM C3NeF IN NORMALS AND PATIENTS WITH MEMBRANO-PROLIFERATIVE GLOMERULONEPHRITIS (MPGN)" @default.
- W1986285429 doi "https://doi.org/10.1203/00006450-198504000-01661" @default.
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