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- W1986289110 abstract "A sub-gene encoding the lipoyl domain (residues 1–85) of the lipoate acetyltransferase chain of the pyruvate dehydrogenase complex of Bacillus stearothermophilus was over-expressed in Escherichia coli. Approx. 80% of the domain was unlipoylated but most of the remainder was correctly lipoylated on Lys-42 and could be reductively acetylated by the B stearothermophilus enzyme complex. A small proportion (approx. 4%) of the domain carried an aberrant substituent, possibly an octanoyl group, on Lys-42. The 400 MHz 1H NMR spectra of the lipoylated and unlipoylated domains were essentially identical and closely resembled that of the native lipoyl domain." @default.
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- W1986289110 date "1990-05-21" @default.
- W1986289110 modified "2023-10-17" @default.
- W1986289110 title "Expression in <i>Escherichia coli</i> of a sub-gene encoding the lipoyl domain of the pyruvate dehydrogenase complex of <i>Bacillus stearothermophilus</i>" @default.
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- W1986289110 doi "https://doi.org/10.1016/0014-5793(90)80249-i" @default.
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