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• W1986291491 endingPage "660" @default.
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• W1986291491 abstract "Saturable, high-affinity binding of iodinated toxin gamma from Tityus serrulatus scorpion venom (TiTx gamma) to Na+ channel receptor was identified in sarcolemma membrane of chick heart. A binding capacity of 450-600 fmol/mg of protein was found similar to that of tetrodotoxin-binding component. The enrichment of these membrane-bound toxin binding sites follows that of other sarcolemma markers. Kinetic data and displacement of 125I-TiTx gamma from its binding sites by unlabeled TiTx gamma gave an equilibrium dissociation constant (Kd) of 1-3 pM. The gating component and the selectivity filter of the voltage-sensitive Na+ channel, identified as binding sites of TiTx gamma and of tetrodotoxin respectively, have been efficiently solubilized with Nonidet P-40. Purification was achieved by ion-exchange chromatography on DEAE-Sephadex A-25, affinity chromatography on wheat-germ-agglutinin-Sepharose and sucrose density gradient centrifugation. An enrichment of 1400-fold from the original detergent extract was measured for both toxin binding sites (1120-1230 pmol/mg of protein). Sodium dodecyl sulfate gel electrophoresis reveals a single large polypeptide component of Mr230000-270000. The purified material exhibits an apparent sedimentation coefficient of 8.8S. Covalent cross-linking of 125I-TiTx gamma to its membrane-embedded cardiac receptor shows that the cross-linked material, solubilized and purified by the same procedure comprises a single polypeptide chain of the same Mr of 230000-270000. Furthermore, as seen for Electrophorus electricus electroplax and rat brain, the tetrodotoxin-binding component and the TiTx gamma-binding component are carried by the same polypeptide chain. The functional Na+ channel might be an oligomer of this subunit of Mr23000-270000." @default.
• W1986291491 created "2016-06-24" @default.
• W1986291491 creator A5064090976 @default.
• W1986291491 creator A5068045779 @default.
• W1986291491 date "1984-06-01" @default.
• W1986291491 modified "2023-09-23" @default.
• W1986291491 title "Characterization, solubilization, affinity labeling and purification of the cardiac Na+ channel using Tityus toxin gamma" @default.
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• W1986291491 doi "https://doi.org/10.1111/j.1432-1033.1984.tb08241.x" @default.
• W1986291491 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6086336" @default.
• W1986291491 hasPublicationYear "1984" @default.
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