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• W1986302514 endingPage "3336" @default.
• W1986302514 startingPage "3325" @default.
• W1986302514 abstract "During endochondral ossification, growth plate chondrocytes release plasma membrane (PM) derived matrix vesicles (MV), which are the site of initial hydroxyapatite crystal formation. MV constituents which facilitate the mineralization process include the integral membrane ectoenzymes alkaline phosphatase (ALPase) and nucleotide pyrophosphatase phosphodiesterase (NPP1/PC-1), along with a phosphatidylserine- (PS-) rich membrane surface that binds annexins and calcium, resulting in enhanced calcium entry into MV. In this study, we determined that chick growth plate MV were highly enriched in membrane raft microdomains containing high levels of cholesterol, glycophosphatidylinositol- (GPI-) anchored ALPase, and phosphatidylserine (PS) localized to the external leaflet of the bilayer. To determine how such membrane microdomains arise during chondrocyte maturation, we explored the role of PM cholesterol-dependent lipid assemblies in regulating the activities of lipid translocators involved in the externalization of PS. We first isolated and determined the composition of detergent-resistant membranes (DRMs) from chondrocyte PM. DRMs isolated from chondrocyte PM were enhanced in ganglioside 1 (GM1) and cholesterol as well as GPI-anchored ALPase. Furthermore, these membrane domains were enriched in PS (localized to the external leaflet of the bilayer) and had significantly higher ALPase activity than non-cholesterol-enriched domains. To understand the role of cholesterol-dependent lipid assemblies in the externalization of PS, we measured the activities of two lipid transporters involved in PS externalization, aminophospholipid translocase (APLT) and phospholipid scramblase (PLSCR1), during maturation of a murine chondrocytic cell line, N1511. In this report, we provide the first evidence that maturing chondrocytes express PLSCR1 and have scramblase activity. We propose that redistribution of PS is dependent on an increase in phospholipid scramblase activity and a decrease in APLT activity. Lastly, we show that translocator activity is most likely to be modulated by membrane cholesterol levels through a membrane raft microdomain." @default.
• W1986302514 created "2016-06-24" @default.
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• W1986302514 date "2006-02-18" @default.
• W1986302514 modified "2023-10-03" @default.
• W1986302514 title "Chondrocytes Utilize a Cholesterol-Dependent Lipid Translocator To Externalize Phosphatidylserine" @default.
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• W1986302514 cites W1593965037 @default.
• W1986302514 cites W178467573 @default.
• W1986302514 cites W181616004 @default.
• W1986302514 cites W1845375977 @default.
• W1986302514 cites W1914926542 @default.
• W1986302514 cites W1946263363 @default.
• W1986302514 cites W1963870861 @default.
• W1986302514 cites W1965192990 @default.
• W1986302514 cites W1969870871 @default.
• W1986302514 cites W1971416162 @default.
• W1986302514 cites W1971717131 @default.
• W1986302514 cites W1972217672 @default.
• W1986302514 cites W1973689937 @default.
• W1986302514 cites W1976455495 @default.
• W1986302514 cites W1979154802 @default.
• W1986302514 cites W1980334501 @default.
• W1986302514 cites W1993357003 @default.
• W1986302514 cites W1993875060 @default.
• W1986302514 cites W2004793282 @default.
• W1986302514 cites W2013839625 @default.
• W1986302514 cites W2017688904 @default.
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• W1986302514 cites W2021691735 @default.
• W1986302514 cites W2021882008 @default.
• W1986302514 cites W2028580514 @default.
• W1986302514 cites W2034718251 @default.
• W1986302514 cites W2036997712 @default.
• W1986302514 cites W2039693127 @default.
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• W1986302514 cites W2052291773 @default.
• W1986302514 cites W2052507797 @default.
• W1986302514 cites W2053204073 @default.
• W1986302514 cites W2056194352 @default.
• W1986302514 cites W2058460324 @default.
• W1986302514 cites W2065886456 @default.
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• W1986302514 cites W2066887588 @default.
• W1986302514 cites W2068908631 @default.
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• W1986302514 cites W2130388575 @default.
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• W1986302514 cites W2147604626 @default.
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• W1986302514 doi "https://doi.org/10.1021/bi0515927" @default.
• W1986302514 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4732727" @default.
• W1986302514 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16519527" @default.
• W1986302514 hasPublicationYear "2006" @default.
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