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• W1986316867 abstract "Abstract Fundamental research of factors affecting cell growth in hollow‐fiber bioreactors is hindered by the lack of an efficient screening tool. To address this issue, a hollow‐fiber micro‐bioreactor has been developed. Hollow fibers with 10 kDa molecular weight cutoffs are housed within a piece of silicone tubing. Cells are inoculated within the hollow fibers which provides a 0.2‐mL culture volume. The space between the fibers and silicone tubing (5 or 16 mL) is used as a medium reservoir sufficient to feed the cells for at least 24 h. Oxygenation is provided directly through the silicone tubing so that a pump for medium recirculation is not required. As a result, many conditions can be tested simultaneously in a single incubator. Three days after inoculation at 5 × 10 6 cells/mL in the micro‐bioreactor, the rho 1D4 murine hybridoma cell line reached 2.8 × 10 7 cells/mL with an antibody concentration of 0.17 mg/mL. When inoculated at 5 × 10 7 cells/mL, the cell concentration reached 1.8 × 10 8 /mL after 3 days with an antibody concentration of 1.0 mg/mL. Results from a series of experiments with the micro‐bioreactor suggested that the initial growth phase of this cell line in a hollow‐fiber system is dependent on the serum concentration in the medium reservoir. This prediction was tested by simultaneously inoculating two production‐scale hollow‐fiber bioreactor systems. The cell side of the membrane for each bioreactor contained 10% serum, but serum was added to the reservoir side of only one of the bioreactors. The cells with only basal medium in the reservoir died after a few days, while the cells with 10% serum in the medium reservoir grew rapidly. These results demonstrate that the micro‐bioreactor developed here can support good cell growth and that it can be used as a research tool to predict the performance of large‐scale hollow‐fiber systems." @default.
• W1986316867 created "2016-06-24" @default.
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• W1986316867 date "1998-01-01" @default.
• W1986316867 modified "2023-10-12" @default.
• W1986316867 title "Screening Tool for Hollow-Fiber Bioreactor Process Development" @default.
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• W1986316867 doi "https://doi.org/10.1021/bp9800081" @default.
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