Matches in SemOpenAlex for { <https://semopenalex.org/work/W1986328639> ?p ?o ?g. }
- W1986328639 endingPage "335" @default.
- W1986328639 startingPage "324" @default.
- W1986328639 abstract "We have characterized an activity in sea urchin eggs which prevents microtubule assembly at minus ends. Using Chlamydomonas axoneme fragments to nucleate the assembly of plus and minus end microtubules, we find robust assembly at microtubule plus ends with negligible assembly at minus ends. The minus end assembly inhibitor does not co-pellet with microtubules when assembly is stimulated with DMSO while the resulting pellet of tubulin and microtubule associated proteins readily assembles from both plus and minus ends of axoneme fragments. Addition of increasing concentrations of porcine brain tubulin to the tubulin and MAP-depleted fraction eventually saturates the minus end inhibitory activity. Compared to purified tubulin, cytosolic fractions both increase the minus end critical concentration ∼3 fold and decrease the plus end critical concentration. The inhibitory activity is removed by heating, trypsin, or by co-immunoprecipitation with tubulin. We hypothesize that a tubulin dimer binding protein is responsible for preventing assembly onto minus ends in our in vitro assays and speculate that this protein functions in vivo to prevent spontaneous nucleation, thus limiting assembly to nucleation sites. © 1996 Wiley-Liss, Inc." @default.
- W1986328639 created "2016-06-24" @default.
- W1986328639 creator A5032938370 @default.
- W1986328639 creator A5041152213 @default.
- W1986328639 date "1996-01-01" @default.
- W1986328639 modified "2023-09-27" @default.
- W1986328639 title "Mechanisms blocking microtubule minus end assembly: Evidence for a tubulin dimer-binding protein" @default.
- W1986328639 cites W1121838620 @default.
- W1986328639 cites W1186643035 @default.
- W1986328639 cites W1510284231 @default.
- W1986328639 cites W1528602163 @default.
- W1986328639 cites W1556021718 @default.
- W1986328639 cites W1579399439 @default.
- W1986328639 cites W1589194753 @default.
- W1986328639 cites W1910135168 @default.
- W1986328639 cites W1963659783 @default.
- W1986328639 cites W1965461114 @default.
- W1986328639 cites W1970611771 @default.
- W1986328639 cites W1974690312 @default.
- W1986328639 cites W1978567246 @default.
- W1986328639 cites W1982401695 @default.
- W1986328639 cites W1990839929 @default.
- W1986328639 cites W1994336523 @default.
- W1986328639 cites W1999974052 @default.
- W1986328639 cites W2002364511 @default.
- W1986328639 cites W2007785335 @default.
- W1986328639 cites W2009785870 @default.
- W1986328639 cites W2015005644 @default.
- W1986328639 cites W2016850953 @default.
- W1986328639 cites W2032920533 @default.
- W1986328639 cites W2034140820 @default.
- W1986328639 cites W2037094686 @default.
- W1986328639 cites W2039303292 @default.
- W1986328639 cites W2040021159 @default.
- W1986328639 cites W2042007591 @default.
- W1986328639 cites W2075734600 @default.
- W1986328639 cites W2076510352 @default.
- W1986328639 cites W2076521022 @default.
- W1986328639 cites W2076659391 @default.
- W1986328639 cites W2082088320 @default.
- W1986328639 cites W2085263653 @default.
- W1986328639 cites W2085357320 @default.
- W1986328639 cites W2087140195 @default.
- W1986328639 cites W2090618328 @default.
- W1986328639 cites W2106672185 @default.
- W1986328639 cites W2116831014 @default.
- W1986328639 cites W2117972095 @default.
- W1986328639 cites W2119460480 @default.
- W1986328639 cites W2121541121 @default.
- W1986328639 cites W2126130533 @default.
- W1986328639 cites W2126462805 @default.
- W1986328639 cites W2127263882 @default.
- W1986328639 cites W2150700233 @default.
- W1986328639 cites W2153128242 @default.
- W1986328639 cites W2155768517 @default.
- W1986328639 cites W2160923065 @default.
- W1986328639 cites W2163658683 @default.
- W1986328639 cites W2166792139 @default.
- W1986328639 cites W2178522090 @default.
- W1986328639 cites W2188467511 @default.
- W1986328639 cites W2215473923 @default.
- W1986328639 cites W2218780553 @default.
- W1986328639 cites W2408318548 @default.
- W1986328639 cites W4293247451 @default.
- W1986328639 doi "https://doi.org/10.1002/(sici)1097-0169(1996)34:4<324::aid-cm7>3.0.co;2-7" @default.
- W1986328639 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8871819" @default.
- W1986328639 hasPublicationYear "1996" @default.
- W1986328639 type Work @default.
- W1986328639 sameAs 1986328639 @default.
- W1986328639 citedByCount "8" @default.
- W1986328639 crossrefType "journal-article" @default.
- W1986328639 hasAuthorship W1986328639A5032938370 @default.
- W1986328639 hasAuthorship W1986328639A5041152213 @default.
- W1986328639 hasConcept C104317684 @default.
- W1986328639 hasConcept C115811362 @default.
- W1986328639 hasConcept C12554922 @default.
- W1986328639 hasConcept C142669718 @default.
- W1986328639 hasConcept C1491633281 @default.
- W1986328639 hasConcept C178790620 @default.
- W1986328639 hasConcept C185592680 @default.
- W1986328639 hasConcept C20418707 @default.
- W1986328639 hasConcept C24870808 @default.
- W1986328639 hasConcept C2779546866 @default.
- W1986328639 hasConcept C55493867 @default.
- W1986328639 hasConcept C61081601 @default.
- W1986328639 hasConcept C71829478 @default.
- W1986328639 hasConcept C84425145 @default.
- W1986328639 hasConcept C86803240 @default.
- W1986328639 hasConcept C95444343 @default.
- W1986328639 hasConceptScore W1986328639C104317684 @default.
- W1986328639 hasConceptScore W1986328639C115811362 @default.
- W1986328639 hasConceptScore W1986328639C12554922 @default.
- W1986328639 hasConceptScore W1986328639C142669718 @default.
- W1986328639 hasConceptScore W1986328639C1491633281 @default.
- W1986328639 hasConceptScore W1986328639C178790620 @default.
- W1986328639 hasConceptScore W1986328639C185592680 @default.
- W1986328639 hasConceptScore W1986328639C20418707 @default.
- W1986328639 hasConceptScore W1986328639C24870808 @default.