Matches in SemOpenAlex for { <https://semopenalex.org/work/W1986357712> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W1986357712 endingPage "1514" @default.
- W1986357712 startingPage "1510" @default.
- W1986357712 abstract "BACKGROUND: Although antibodies to Js a and Js b are clinically significant, reagent‐quality anti‐Js a and anti‐Js b are not readily available. A sequence‐specific primer–polymerase chain reaction (SSP‐PCR) genotyping assay was tested that makes use of two single‐nucleotide polymorphisms (SNPs) at positions 1910 and 2019 of KEL . These SNPs distinguish the gene encoding Js a , KEL6; and Js b , KEL7 . STUDY DESIGN AND METHODS: Four primer sets that selectively amplified KEL6 and KEL7 from genomic DNA were developed. Two sets detected the SNP at bp 1910 and two sets detected the bp 2019 SNP. KEL6 and KEL7 genotyping and Js a and Js b phenotyping results were compared among 64 subjects. RESULTS: The SSP‐PCRs were specific for KEL6 and KEL7 when testing DNA for three donors of known Js phenotype: Js(a+b–), Js(a–b+), and Js(a+b+). Genotyping results for the 1910 SNP were identical to the phenotyping results in all 64 subjects, but for the 2019 SNP, the genotyping and phenotyping results were identical for only 49 subjects. In 12 subjects with the Js(a–b+) phenotype, the 2019 SNP was heterozygous KEL6 , KEL7; in 2 with Js(a–b+) and in 1 with Js(a+b+), the 2019 SNP was homozygous KEL6 . CONCLUSION: KEL 2019‐bp SNP does not always correlate with the Js phenotype owing to the presence of an atypical KEL gene with a KEL7 polymorphism at 1910 and a KEL6 polymorphism at 2019. The KEL polymorphism at 2019 is silent and this allele yields a Js(a–b+) phenotype. Only analysis of the 1910‐bp SNP can be used to genotype KEL6 and KEL7 ." @default.
- W1986357712 created "2016-06-24" @default.
- W1986357712 creator A5001407294 @default.
- W1986357712 creator A5001814795 @default.
- W1986357712 creator A5002629732 @default.
- W1986357712 creator A5072166479 @default.
- W1986357712 creator A5072275854 @default.
- W1986357712 creator A5087179097 @default.
- W1986357712 creator A5090423342 @default.
- W1986357712 date "2006-09-01" @default.
- W1986357712 modified "2023-10-15" @default.
- W1986357712 title "KEL6 and KEL7 genotyping with sequence-specific primers" @default.
- W1986357712 cites W121403798 @default.
- W1986357712 cites W1982666857 @default.
- W1986357712 cites W2033167472 @default.
- W1986357712 cites W2045338804 @default.
- W1986357712 cites W2047130270 @default.
- W1986357712 cites W2060869850 @default.
- W1986357712 cites W2066708380 @default.
- W1986357712 cites W2069535861 @default.
- W1986357712 cites W2097753206 @default.
- W1986357712 cites W2146840490 @default.
- W1986357712 cites W2158691700 @default.
- W1986357712 cites W2419385606 @default.
- W1986357712 doi "https://doi.org/10.1111/j.1537-2995.2006.00937.x" @default.
- W1986357712 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16965577" @default.
- W1986357712 hasPublicationYear "2006" @default.
- W1986357712 type Work @default.
- W1986357712 sameAs 1986357712 @default.
- W1986357712 citedByCount "5" @default.
- W1986357712 countsByYear W19863577122013 @default.
- W1986357712 countsByYear W19863577122014 @default.
- W1986357712 countsByYear W19863577122020 @default.
- W1986357712 crossrefType "journal-article" @default.
- W1986357712 hasAuthorship W1986357712A5001407294 @default.
- W1986357712 hasAuthorship W1986357712A5001814795 @default.
- W1986357712 hasAuthorship W1986357712A5002629732 @default.
- W1986357712 hasAuthorship W1986357712A5072166479 @default.
- W1986357712 hasAuthorship W1986357712A5072275854 @default.
- W1986357712 hasAuthorship W1986357712A5087179097 @default.
- W1986357712 hasAuthorship W1986357712A5090423342 @default.
- W1986357712 hasConcept C104317684 @default.
- W1986357712 hasConcept C135763542 @default.
- W1986357712 hasConcept C139275648 @default.
- W1986357712 hasConcept C149737253 @default.
- W1986357712 hasConcept C153209595 @default.
- W1986357712 hasConcept C153911025 @default.
- W1986357712 hasConcept C163691529 @default.
- W1986357712 hasConcept C178790620 @default.
- W1986357712 hasConcept C185592680 @default.
- W1986357712 hasConcept C2777563447 @default.
- W1986357712 hasConcept C31467283 @default.
- W1986357712 hasConcept C49105822 @default.
- W1986357712 hasConcept C54355233 @default.
- W1986357712 hasConcept C59701697 @default.
- W1986357712 hasConcept C86803240 @default.
- W1986357712 hasConceptScore W1986357712C104317684 @default.
- W1986357712 hasConceptScore W1986357712C135763542 @default.
- W1986357712 hasConceptScore W1986357712C139275648 @default.
- W1986357712 hasConceptScore W1986357712C149737253 @default.
- W1986357712 hasConceptScore W1986357712C153209595 @default.
- W1986357712 hasConceptScore W1986357712C153911025 @default.
- W1986357712 hasConceptScore W1986357712C163691529 @default.
- W1986357712 hasConceptScore W1986357712C178790620 @default.
- W1986357712 hasConceptScore W1986357712C185592680 @default.
- W1986357712 hasConceptScore W1986357712C2777563447 @default.
- W1986357712 hasConceptScore W1986357712C31467283 @default.
- W1986357712 hasConceptScore W1986357712C49105822 @default.
- W1986357712 hasConceptScore W1986357712C54355233 @default.
- W1986357712 hasConceptScore W1986357712C59701697 @default.
- W1986357712 hasConceptScore W1986357712C86803240 @default.
- W1986357712 hasIssue "9" @default.
- W1986357712 hasLocation W19863577121 @default.
- W1986357712 hasLocation W19863577122 @default.
- W1986357712 hasOpenAccess W1986357712 @default.
- W1986357712 hasPrimaryLocation W19863577121 @default.
- W1986357712 hasRelatedWork W2058157972 @default.
- W1986357712 hasRelatedWork W2098610461 @default.
- W1986357712 hasRelatedWork W2104756498 @default.
- W1986357712 hasRelatedWork W2145636404 @default.
- W1986357712 hasRelatedWork W2202951024 @default.
- W1986357712 hasRelatedWork W2414446413 @default.
- W1986357712 hasRelatedWork W2936024166 @default.
- W1986357712 hasRelatedWork W2970780691 @default.
- W1986357712 hasRelatedWork W3210640461 @default.
- W1986357712 hasRelatedWork W3217112812 @default.
- W1986357712 hasVolume "46" @default.
- W1986357712 isParatext "false" @default.
- W1986357712 isRetracted "false" @default.
- W1986357712 magId "1986357712" @default.
- W1986357712 workType "article" @default.