Matches in SemOpenAlex for { <https://semopenalex.org/work/W1986363532> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W1986363532 endingPage "298" @default.
- W1986363532 startingPage "285" @default.
- W1986363532 abstract "Ifosfamide (IF) is an alkylating cytostatic drug with urotoxic (hemorrhagic cystitis) and nephrotoxic side effects. Several cases of Fanconi syndrome in children following therapy with IF were reported. Little information is available concerning the pathomechanisms of transport inhibition by IF. We used a permanent renal epithelial cell line with proximal tubular characteristics (LLC-PK1) in order to investigate the effects of IF and some of its major metabolites (4-OH-IF, chloracetaldehyde, and acrolein). LLC-PK1 cells were used in a confluent state. Sodium-dependent and sodium-independent fluxes of 32PO4 were determined by standard techniques. Activities of marker enzymes of apical and basolateral membranes, of mitochondria, and of endoplasmic reticulum were determined in cell homogenates. IF induces a moderate stimulation of PO4 transport. 4-OH-IF also has a stimulatory effect on transport at low concentrations (up to 200 mumol/l) and with short incubation (2h), while a 24-hour exposure of cells to 100 mumol/l of 4-OH-IF has an inhibitory effect of PO4 transport. Concentrations of 4-OH-IF which inhibit transport also reduce the activity of Na(+)-K(+)-ATPase. Chloracetaldehyde, like 4-OH-IF, induces a biphasic response of PO4 transport with stimulation in the low concentration range (up to 75 mumol/l) and inhibition at higher concentrations. Chloracetaldehyde reduces the activity of succinate-cytochrome c oxidoreductase, suggesting that a defect in ATP generation might play a role in the pathogenesis of Fanconi syndrome induced by IF. Acrolein strongly damages monolayers and reduces sodium-dependent transport of PO4 to very low levels at 150 mumol/l. It reduces the activities of both Na(+)-K+ ATPase and succinate-cytochrome c oxidoreductase. Acrolein also is the only metabolite with a moderate effect on alkaline phosphatase. We conclude that sodium-dependent transport of PO4 is highly sensitive to IF metabolites. In addition to direct toxic effects of IF metabolites on transport proteins within the apical plasma membrane, damage to mitochondrial enzymes and to Na(+)-K+ ATPase which generates the electrochemical gradients for secondary active PO4 transport may play an important role in the pathogenesis of Fanconi syndrome induced by IF." @default.
- W1986363532 created "2016-06-24" @default.
- W1986363532 creator A5006340457 @default.
- W1986363532 creator A5034749844 @default.
- W1986363532 creator A5037496962 @default.
- W1986363532 creator A5051019486 @default.
- W1986363532 creator A5085318712 @default.
- W1986363532 date "1993-01-01" @default.
- W1986363532 modified "2023-09-26" @default.
- W1986363532 title "Effect of Ifosfamide Metabolites on Sodium-Dependent Phosphate Transport in a Model of Proximal Tubular Cells (LLC-PK<sub>1</sub>) in Culture" @default.
- W1986363532 doi "https://doi.org/10.1159/000173775" @default.
- W1986363532 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7506438" @default.
- W1986363532 hasPublicationYear "1993" @default.
- W1986363532 type Work @default.
- W1986363532 sameAs 1986363532 @default.
- W1986363532 citedByCount "7" @default.
- W1986363532 countsByYear W19863635322014 @default.
- W1986363532 countsByYear W19863635322015 @default.
- W1986363532 countsByYear W19863635322019 @default.
- W1986363532 crossrefType "journal-article" @default.
- W1986363532 hasAuthorship W1986363532A5006340457 @default.
- W1986363532 hasAuthorship W1986363532A5034749844 @default.
- W1986363532 hasAuthorship W1986363532A5037496962 @default.
- W1986363532 hasAuthorship W1986363532A5051019486 @default.
- W1986363532 hasAuthorship W1986363532A5085318712 @default.
- W1986363532 hasConcept C126189478 @default.
- W1986363532 hasConcept C126322002 @default.
- W1986363532 hasConcept C134018914 @default.
- W1986363532 hasConcept C158617107 @default.
- W1986363532 hasConcept C178790620 @default.
- W1986363532 hasConcept C185592680 @default.
- W1986363532 hasConcept C2776694085 @default.
- W1986363532 hasConcept C2777506904 @default.
- W1986363532 hasConcept C2778239845 @default.
- W1986363532 hasConcept C2779026464 @default.
- W1986363532 hasConcept C2780091579 @default.
- W1986363532 hasConcept C29730261 @default.
- W1986363532 hasConcept C537181965 @default.
- W1986363532 hasConcept C54355233 @default.
- W1986363532 hasConcept C55493867 @default.
- W1986363532 hasConcept C71924100 @default.
- W1986363532 hasConcept C81885089 @default.
- W1986363532 hasConcept C86803240 @default.
- W1986363532 hasConceptScore W1986363532C126189478 @default.
- W1986363532 hasConceptScore W1986363532C126322002 @default.
- W1986363532 hasConceptScore W1986363532C134018914 @default.
- W1986363532 hasConceptScore W1986363532C158617107 @default.
- W1986363532 hasConceptScore W1986363532C178790620 @default.
- W1986363532 hasConceptScore W1986363532C185592680 @default.
- W1986363532 hasConceptScore W1986363532C2776694085 @default.
- W1986363532 hasConceptScore W1986363532C2777506904 @default.
- W1986363532 hasConceptScore W1986363532C2778239845 @default.
- W1986363532 hasConceptScore W1986363532C2779026464 @default.
- W1986363532 hasConceptScore W1986363532C2780091579 @default.
- W1986363532 hasConceptScore W1986363532C29730261 @default.
- W1986363532 hasConceptScore W1986363532C537181965 @default.
- W1986363532 hasConceptScore W1986363532C54355233 @default.
- W1986363532 hasConceptScore W1986363532C55493867 @default.
- W1986363532 hasConceptScore W1986363532C71924100 @default.
- W1986363532 hasConceptScore W1986363532C81885089 @default.
- W1986363532 hasConceptScore W1986363532C86803240 @default.
- W1986363532 hasIssue "6" @default.
- W1986363532 hasLocation W19863635321 @default.
- W1986363532 hasLocation W19863635322 @default.
- W1986363532 hasOpenAccess W1986363532 @default.
- W1986363532 hasPrimaryLocation W19863635321 @default.
- W1986363532 hasRelatedWork W107164118 @default.
- W1986363532 hasRelatedWork W1803392829 @default.
- W1986363532 hasRelatedWork W1935082605 @default.
- W1986363532 hasRelatedWork W1992092198 @default.
- W1986363532 hasRelatedWork W1994848669 @default.
- W1986363532 hasRelatedWork W2024302689 @default.
- W1986363532 hasRelatedWork W2028342916 @default.
- W1986363532 hasRelatedWork W2116874784 @default.
- W1986363532 hasRelatedWork W2441865394 @default.
- W1986363532 hasRelatedWork W4243113070 @default.
- W1986363532 hasVolume "16" @default.
- W1986363532 isParatext "false" @default.
- W1986363532 isRetracted "false" @default.
- W1986363532 magId "1986363532" @default.
- W1986363532 workType "article" @default.