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• W1986440895 abstract "ABSTRACT Thirty-two vanB glycopeptide-resistant enterococci (28 Enterococcus faecium and 4 Enterococcus faecalis ) were collected from hospitalized patients in Glasgow, Edinburgh, Dundee, and Aberdeen, Scotland, and the vanB element in each was compared to vanB1 of E. faecalis strain ATCC 51299. Hha I digestion of PCR fragments of the vanB ligase gene was used to identify vanB subtypes. All E. faecium isolates were vanB2 , and all E. faecalis isolates were vanB1 . Restriction fragment length polymorphism analysis of a 5,180-bp vanS B -vanX B long-PCR fragment of the vanB cluster showed the loss of Hae II restriction sites in vanS B , vanW , and vanX B in strains containing a vanB2 ligase gene. Partial sequences of genes in the vanB2 cluster for two genomically distinct Scottish isolates were >99.8% identical to each other. vanS B2 , vanX B2 , and vanB2 sequences differed at the nucleotide level from those of vanS B , vanX B , and vanB by 4.2, 4.6, and 4.8%, respectively. The vanB2 resistance element appears to be widespread among VanB glycopeptide-resistant E. faecium strains isolated in Scottish hospitals." @default.
• W1986440895 created "2016-06-24" @default.
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• W1986440895 date "2000-09-01" @default.
• W1986440895 modified "2023-10-17" @default.
• W1986440895 title "Identification and Characterization of vanB2 Glycopeptide Resistance Elements in Enterococci Isolated in Scotland" @default.
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• W1986440895 doi "https://doi.org/10.1128/aac.44.9.2341-2348.2000" @default.
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