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• W1986482015 abstract "Abstract The aim of this study was to investigate the effect of TiO 2 scaffold (SC) coated with an alginate hydrogel containing a proline‐rich peptide (P2) on osteoblast proliferation and differentiation in vitro . Peptide release was evaluated and a burst release was observed during the first hours of incubation, and then progressively released overtime. No changes were observed in the cytotoxicity after 48 h of seeding MC3T3‐E1 cells on the coated and uncoated TiO 2 SC. The amount of cells after 7 days was higher on uncoated TiO 2 SC than on alginate‐coated TiO 2 SC, measured by DNA content and scanning electron microscope imaging. In addition, while lower expression of integrin beta1 was detected for alginate‐coated TiO 2 SC at this time point, similar gene expression was observed for other integrins, fibronectin‐1, and several osteoblast differentiation markers. After 21 days, gene expression of integrin beta3, fibronectin‐1, osterix, and collagen‐I was increased in alginate‐coated compared to TiO 2 SC. Moreover, increased gene expression of integrin alpha8, bone morphogenetic protein 2, interleukin‐6, and collagen‐I was found on P2 alginate‐coated TiO 2 SC compared to alginate‐coated TiO 2 SC. In conclusion, our results indicate that alginate‐coated TiO 2 SC can act as a matrix for delivery of proline‐rich peptides increasing osteoblast differentiation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2013." @default.
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• W1986482015 date "2012-11-30" @default.
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• W1986482015 title "Effect of TiO₂scaffolds coated with alginate hydrogel containing a proline-rich peptide on osteoblast growth and differentiation<i>in vitro</i>" @default.
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• W1986482015 doi "https://doi.org/10.1002/jbm.a.34458" @default.
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