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• W1986514145 abstract "Microemulsions composed of a monolayer of dimyristoyl phosphatidylcholine enclosing a core of cholesterol oleate have been characterized with respect to size and the physical state of the monolayer lipid. Fluorescent and spin-labeled fatty acids (n-(9-anthroyloxy) stearates and n-doxyl stearates, respectively) have been used to examine the fluidity and the order at several depths within the monolayer. Below the phase transition of the phospholipid, the emulsion monolayer is more fluid than the vesicle bilayer composed of the same phospholipid. Above the phase transition, the bilayer is the more fluid structure. The phase transition of the surface monolayer in the emulsion is significantly broadened compared to the sharp transition which is characteristic of the lipid bilayer. The broadening is not an intrinsic characteristic of the monolayer, nor is it due to small amounts of cholesterol ester soluble in the monolayer. The broadening can be attributed to a disruption of the lipid packing at the monolayer-core interface or to difficulty in accommodating changes in the molar volume of the phospholipid through the transition. The use of fluorescence quenching techniques to quantitatively determine the partition of cholesterol esters between the monolayer and core compartments is described and the limitations of this technique are discussed." @default.
• W1986514145 created "2016-06-24" @default.
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• W1986514145 date "1990-01-01" @default.
• W1986514145 modified "2023-09-24" @default.
• W1986514145 title "Structure and dynamics of microemulsions which mimic the lipid phase of low-density lipoproteins" @default.
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• W1986514145 doi "https://doi.org/10.1016/0005-2760(90)90054-2" @default.
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