FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1986585752> ?p ?o ?g. }

• W1986585752 endingPage "79" @default.
• W1986585752 startingPage "69" @default.
• W1986585752 abstract "A two-domain portion of the proteinase inhibitor precursor from Nicotiana alata (NaProPI) has been expressed and its structure determined by NMR spectroscopy. NaProPI contains six almost identical 53 amino acid repeats that fold into six highly similar domains; however, the sequence repeats do not coincide with the structural domains. Five of the structural domains comprise the C-terminal portion of one repeat and the N-terminal portion of the next. The sixth domain contains the C-terminal portion of the sixth repeat and the N-terminal portion of the first repeat. Disulphide bonds link these C and N-terminal fragments to generate the clasped-bracelet fold of NaProPI. The three-dimensional structure of NaProPI is not known, but it is conceivable that adjacent domains in NaProPI interact to generate the circular “bracelet” with the N and C termini in close enough proximity to facilitate formation of the disulphide bonds that form the “clasp”. The expressed protein, examined in the current study, comprises residues 25–135 of NaProPI and encompasses the first two contiguous structural domains, namely the chymotrypsin inhibitor C1 and the trypsin inhibitor T1, joined by a five-residue linker, and is referred to as C1-T1. The tertiary structure of each domain in C1-T1 is identical to that found in the isolated inhibitors. However, no nuclear Overhauser effect contacts are observed between the two domains and the five-residue linker adopts an extended conformation. The absence of interactions between the domains indicates that adjacent domains do not specifically interact to drive the circularisation of NaProPI. These results are in agreement with recent data which describe similar PI precursors from other members of the Solanaceae having two, three, or four repeats. The lack of strong interdomain association is likely to be important for the function of individual inhibitors by ensuring that there is no masking of reactive sites upon release from the precursor." @default.
• W1986585752 created "2016-06-24" @default.
• W1986585752 creator A5013189928 @default.
• W1986585752 creator A5037467309 @default.
• W1986585752 creator A5040684603 @default.
• W1986585752 creator A5050405858 @default.
• W1986585752 creator A5079308758 @default.
• W1986585752 date "2001-02-01" @default.
• W1986585752 modified "2023-09-27" @default.
• W1986585752 title "The solution structure of C1-T1, a two-domain proteinase inhibitor derived from a circular precursor protein from Nicotiana alata11Edited by P. E. Wright" @default.
• W1986585752 cites W1561454246 @default.
• W1986585752 cites W1579109316 @default.
• W1986585752 cites W1971966571 @default.
• W1986585752 cites W1975459052 @default.
• W1986585752 cites W1986322598 @default.
• W1986585752 cites W1987968622 @default.
• W1986585752 cites W1988542675 @default.
• W1986585752 cites W1993844842 @default.
• W1986585752 cites W2000948557 @default.
• W1986585752 cites W2002195659 @default.
• W1986585752 cites W2003458079 @default.
• W1986585752 cites W2006725068 @default.
• W1986585752 cites W2008344756 @default.
• W1986585752 cites W2016621691 @default.
• W1986585752 cites W2017515564 @default.
• W1986585752 cites W2022902986 @default.
• W1986585752 cites W2024895368 @default.
• W1986585752 cites W2033982014 @default.
• W1986585752 cites W2036454087 @default.
• W1986585752 cites W2040348563 @default.
• W1986585752 cites W2045446885 @default.
• W1986585752 cites W2046060532 @default.
• W1986585752 cites W2052933818 @default.
• W1986585752 cites W2061141805 @default.
• W1986585752 cites W2065300643 @default.
• W1986585752 cites W2077195973 @default.
• W1986585752 cites W2085332399 @default.
• W1986585752 cites W2088848851 @default.
• W1986585752 cites W2088932078 @default.
• W1986585752 cites W2093700493 @default.
• W1986585752 cites W2106013220 @default.
• W1986585752 cites W2107837249 @default.
• W1986585752 cites W2139783945 @default.
• W1986585752 cites W2150496578 @default.
• W1986585752 cites W2151687360 @default.
• W1986585752 cites W2162166182 @default.
• W1986585752 cites W4254975613 @default.
• W1986585752 doi "https://doi.org/10.1006/jmbi.2000.4318" @default.
• W1986585752 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11178894" @default.
• W1986585752 hasPublicationYear "2001" @default.
• W1986585752 type Work @default.
• W1986585752 sameAs 1986585752 @default.
• W1986585752 citedByCount "21" @default.
• W1986585752 countsByYear W19865857522012 @default.
• W1986585752 countsByYear W19865857522013 @default.
• W1986585752 countsByYear W19865857522014 @default.
• W1986585752 countsByYear W19865857522018 @default.
• W1986585752 countsByYear W19865857522019 @default.
• W1986585752 crossrefType "journal-article" @default.
• W1986585752 hasAuthorship W1986585752A5013189928 @default.
• W1986585752 hasAuthorship W1986585752A5037467309 @default.
• W1986585752 hasAuthorship W1986585752A5040684603 @default.
• W1986585752 hasAuthorship W1986585752A5050405858 @default.
• W1986585752 hasAuthorship W1986585752A5079308758 @default.
• W1986585752 hasConcept C111919701 @default.
• W1986585752 hasConcept C133571119 @default.
• W1986585752 hasConcept C181199279 @default.
• W1986585752 hasConcept C185592680 @default.
• W1986585752 hasConcept C2778401398 @default.
• W1986585752 hasConcept C2780340462 @default.
• W1986585752 hasConcept C2780557392 @default.
• W1986585752 hasConcept C2781338088 @default.
• W1986585752 hasConcept C3018687534 @default.
• W1986585752 hasConcept C41008148 @default.
• W1986585752 hasConcept C47701112 @default.
• W1986585752 hasConcept C515207424 @default.
• W1986585752 hasConcept C55493867 @default.
• W1986585752 hasConcept C57711820 @default.
• W1986585752 hasConcept C66974803 @default.
• W1986585752 hasConcept C71240020 @default.
• W1986585752 hasConcept C8010536 @default.
• W1986585752 hasConceptScore W1986585752C111919701 @default.
• W1986585752 hasConceptScore W1986585752C133571119 @default.
• W1986585752 hasConceptScore W1986585752C181199279 @default.
• W1986585752 hasConceptScore W1986585752C185592680 @default.
• W1986585752 hasConceptScore W1986585752C2778401398 @default.
• W1986585752 hasConceptScore W1986585752C2780340462 @default.
• W1986585752 hasConceptScore W1986585752C2780557392 @default.
• W1986585752 hasConceptScore W1986585752C2781338088 @default.
• W1986585752 hasConceptScore W1986585752C3018687534 @default.
• W1986585752 hasConceptScore W1986585752C41008148 @default.
• W1986585752 hasConceptScore W1986585752C47701112 @default.
• W1986585752 hasConceptScore W1986585752C515207424 @default.
• W1986585752 hasConceptScore W1986585752C55493867 @default.
• W1986585752 hasConceptScore W1986585752C57711820 @default.
• W1986585752 hasConceptScore W1986585752C66974803 @default.
• W1986585752 hasConceptScore W1986585752C71240020 @default.
• W1986585752 hasConceptScore W1986585752C8010536 @default.

• next