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• W1986618702 abstract "Virulence genes of the Agrobacterium tumefaciens Ti plasmid are positively regulated by the products of virA and virG. To study the DNA-binding properties of the VirG protein, a translational fusion between virG and the frpEgene of Escherichia coli was constructed, and antiserum was raised against the encoded fusion protein. Using this antiserum, a protein of Mr= 29000, a size similar to that calculated from the virG nucleotide sequence, was detected in an E. coli strain harbouring a virG expression vector. Both the VirG protein and the fusion protein were found, by filter-binding and gel retardation analyses, to bind DNA nonspecifically. These data support an existing model for the two-component regulatory systems of bacteria." @default.
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• W1986618702 date "1989-03-01" @default.
• W1986618702 modified "2023-09-27" @default.
• W1986618702 title "virG of Agrobacterium tumefaciens plasmid pTiC58 encodes a DNA-binding protein" @default.
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• W1986618702 doi "https://doi.org/10.1111/j.1365-2958.1989.tb00186.x" @default.
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