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- W1986654313 abstract "The division cycle and RNA synthesis were analysed in early and late passage cells of a human fibroblastic strain (WI-38). Cells were exposed to tritium labeled thymidine or uridine. Cultures after the 40th passage were found to be very heterogeneous regarding the length of the division cycle. The fraction of cells involved in DNA synthesis after a short pulse was smaller and the generation time was prolonged in late passage cells. Evidence is presented that the cause for a prolonged generation time was mainy due to retardation in the G1 and G2 periods. The rate of synthesis of RNA was found to be decreased in late passage cells. However, the pattern of incorporation of labeled precursor was the same in both types of cells and active RNA synthesis occurred in 100 per cent of the phase III cells." @default.
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- W1986654313 date "1966-06-01" @default.
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- W1986654313 title "The division cycle and RNA-synthesis in diploid human cells at different passage levels in vitro" @default.
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- W1986654313 doi "https://doi.org/10.1016/0014-4827(66)90280-1" @default.
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