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• W1986656144 abstract "The development of a SYBR Green-based duplex real-time PCR is described for simultaneous detection of porcine parvovirus (PPV) and porcine circovirus type 2 (PCV-2) genomes. Viral genomes were identified in the same sample by their distinctive melting temperature (Tm) which is 77.5 °C for PPV VP2 313 bp amplicon and 82.3 °C for PCV-2 ORF2 171 bp amplicon, respectively. The detection limit of the method was 0.01 TCID50/mL for PPV and PCV-2, about 10 times more sensitive than conventional PCR. In addition, PPV and PCV-2 viral load were measured in 126 field samples, confirming the sensitivity and specificity, and the result showed that 70/126 samples were positive for PPV and 92/126 samples were positive for PCV2 by the duplex real-time PCR. This method may be a useful alternative rapid and reliable method for the detection of PPV/PCV-2 co-infection." @default.
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• W1986656144 date "2013-01-01" @default.
• W1986656144 modified "2023-09-26" @default.
• W1986656144 title "Simultaneous detection of porcine parvovirus and porcine circovirus type 2 by duplex real-time PCR and amplicon melting curve analysis using SYBR Green" @default.
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• W1986656144 doi "https://doi.org/10.1016/j.jviromet.2012.06.024" @default.
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