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- W1986683451 abstract "The effect of various stains on the morphology of Rauscher leukemia virus was examined. The virus appeared as a round, membrane-bound structure when prefixed with glutaraldehyde or formalin and stained with uranyl acetate (UA). Other stains, such as sodium phosphotungstate, sodium silicotungstate, or ammonium molybdate caused disruption of over 80% of the virus particles. They then appeared as smaller, tailed particles or swollen, heavily stained membranous structures. In the prefixed, UA-stained preparations, the particles were accurately measured using the catalase crystal internal marker technique. The intact virus had an average diameter of 1470 Å. Also present were smaller particles with an outer diameter of 1090 Å, which occurred at a level of 20–30%. The proportion of these smaller structures could be increased to 70% of the total particles by a brief treatment with 0.01% dodecyl sodium sulfate (SDS). This suggests that the outer envelope of the intact virus was selectively removed, thus exposing an internal nucleoid. This is consistent with the observation that isolated viral outer envelopes measured 200 Å across. The nucleoids obtained by SDS-treatment banded in cesium chloride (CsCl) at an average density of 1.226 g/ml, compared to the value of 1.18 g/ml for the fully enveloped virion, which also suggests the loss of a lipid envelope. Occasionally, nucleoid particles were fortuitously stained to reveal an internal single or double-stranded whorled substructure. The double-stranded form was 2.5 times more prevalent in virus harvested at 3 hr than at 24 hr. The contour length of both whorled forms was about the same and corresponds to a three-dimensional coil 1.1 – 1.5 μ long. Upon prolonged treatment of virus harvested at 3 hr with 0.01% SDS, the whorled structures could be liberated from the nucleoids. They appeared as strands about 1.18 μ long, and banded in CsCl at an average density of 1.343 g/ml. Their localization in the particle by UA staining, as well as their density suggest that these strands are nucleoprotein in nature. Models based on how the strand is packaged in the nucleoid are suggested to explain how the various internal forms could arise in the virus population." @default.
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- W1986683451 date "1971-11-01" @default.
- W1986683451 modified "2023-09-27" @default.
- W1986683451 title "An electron microscope study of Rauscher leukemia virus" @default.
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- W1986683451 doi "https://doi.org/10.1016/0042-6822(71)90030-4" @default.
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