FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1986720797> ?p ?o ?g. }

• W1986720797 endingPage "617" @default.
• W1986720797 startingPage "605" @default.
• W1986720797 abstract "TorsinA is a member of the AAA+ ATPase family of proteins and, notably, is the only known ATPase localized to the ER lumen. It has been suggested to act as a molecular chaperone, while a mutant form associated with early-onset torsion dystonia, a dominantly inherited movement disorder, appears to result in a net loss of function in vivo. Thus far, no studies have examined the chaperone activity of torsinA in vitro. Here we expressed and purified both wild-type (WT) and mutant torsinA fusion proteins in bacteria and examined their ability to function as molecular chaperones by monitoring suppression of luciferase and citrate synthase (CS) aggregation. We also assessed their ability to hold proteins in an intermediate state for refolding. As measured by light scattering and SDS-PAGE, both WT and mutant torsinA effectively, and similarly, suppressed protein aggregation compared to controls. This function was not further enhanced by the presence of ATP. Further, we found that while neither form of torsinA could protect CS from heat-induced inactivation, they were both able to reactivate luciferase when ATP and rabbit reticulocyte lysate were added. This suggests that torsinA holds luciferase in an intermediate state, which can then be refolded in the presence of other chaperones. These data provide conclusive evidence that torsinA acts as a molecular chaperone in vitro and suggests that early-onset torsion dystonia is likely not a consequence of a loss in torsinA chaperone activity but might be an outcome of insufficient torsinA localization at the ER to manage protein folding or trafficking." @default.
• W1986720797 created "2016-06-24" @default.
• W1986720797 creator A5017177287 @default.
• W1986720797 creator A5033267154 @default.
• W1986720797 creator A5066168948 @default.
• W1986720797 creator A5079125985 @default.
• W1986720797 date "2010-02-19" @default.
• W1986720797 modified "2023-10-01" @default.
• W1986720797 title "The early-onset torsion dystonia-associated protein, torsinA, displays molecular chaperone activity in vitro" @default.
• W1986720797 cites W1504141563 @default.
• W1986720797 cites W1505317673 @default.
• W1986720797 cites W1507128123 @default.
• W1986720797 cites W1523616760 @default.
• W1986720797 cites W1537274817 @default.
• W1986720797 cites W161906830 @default.
• W1986720797 cites W1824821953 @default.
• W1986720797 cites W1971497139 @default.
• W1986720797 cites W1982736767 @default.
• W1986720797 cites W1983589934 @default.
• W1986720797 cites W1985183002 @default.
• W1986720797 cites W1986783417 @default.
• W1986720797 cites W1988259487 @default.
• W1986720797 cites W1997720284 @default.
• W1986720797 cites W1998432903 @default.
• W1986720797 cites W1998479277 @default.
• W1986720797 cites W2001730407 @default.
• W1986720797 cites W2017613141 @default.
• W1986720797 cites W2018193494 @default.
• W1986720797 cites W2019037864 @default.
• W1986720797 cites W2019531132 @default.
• W1986720797 cites W2020102061 @default.
• W1986720797 cites W2022038675 @default.
• W1986720797 cites W2035198471 @default.
• W1986720797 cites W2037572295 @default.
• W1986720797 cites W2038347400 @default.
• W1986720797 cites W2043693325 @default.
• W1986720797 cites W2049979242 @default.
• W1986720797 cites W2051323334 @default.
• W1986720797 cites W2055956984 @default.
• W1986720797 cites W2057281288 @default.
• W1986720797 cites W2058363047 @default.
• W1986720797 cites W2060644195 @default.
• W1986720797 cites W2061093994 @default.
• W1986720797 cites W2065146040 @default.
• W1986720797 cites W2066179156 @default.
• W1986720797 cites W2067455339 @default.
• W1986720797 cites W2074802067 @default.
• W1986720797 cites W2075633711 @default.
• W1986720797 cites W2079050436 @default.
• W1986720797 cites W2082520757 @default.
• W1986720797 cites W2083605841 @default.
• W1986720797 cites W2084165516 @default.
• W1986720797 cites W2084752631 @default.
• W1986720797 cites W2088078969 @default.
• W1986720797 cites W2090219621 @default.
• W1986720797 cites W2091151964 @default.
• W1986720797 cites W2098691225 @default.
• W1986720797 cites W2101385560 @default.
• W1986720797 cites W2107801170 @default.
• W1986720797 cites W2118076431 @default.
• W1986720797 cites W2119353017 @default.
• W1986720797 cites W2120869853 @default.
• W1986720797 cites W2122054000 @default.
• W1986720797 cites W2127033103 @default.
• W1986720797 cites W2130566610 @default.
• W1986720797 cites W2133119603 @default.
• W1986720797 cites W2136356801 @default.
• W1986720797 cites W2139232591 @default.
• W1986720797 cites W2150261685 @default.
• W1986720797 cites W2154455082 @default.
• W1986720797 cites W2158565708 @default.
• W1986720797 cites W2162791176 @default.
• W1986720797 cites W2166820447 @default.
• W1986720797 cites W2178835622 @default.
• W1986720797 cites W2206758221 @default.
• W1986720797 doi "https://doi.org/10.1007/s12192-010-0173-2" @default.
• W1986720797 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3006618" @default.
• W1986720797 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20169475" @default.
• W1986720797 hasPublicationYear "2010" @default.
• W1986720797 type Work @default.
• W1986720797 sameAs 1986720797 @default.
• W1986720797 citedByCount "54" @default.
• W1986720797 countsByYear W19867207972012 @default.
• W1986720797 countsByYear W19867207972013 @default.
• W1986720797 countsByYear W19867207972014 @default.
• W1986720797 countsByYear W19867207972015 @default.
• W1986720797 countsByYear W19867207972016 @default.
• W1986720797 countsByYear W19867207972017 @default.
• W1986720797 countsByYear W19867207972018 @default.
• W1986720797 countsByYear W19867207972019 @default.
• W1986720797 countsByYear W19867207972020 @default.
• W1986720797 countsByYear W19867207972021 @default.
• W1986720797 countsByYear W19867207972023 @default.
• W1986720797 crossrefType "journal-article" @default.
• W1986720797 hasAuthorship W1986720797A5017177287 @default.
• W1986720797 hasAuthorship W1986720797A5033267154 @default.
• W1986720797 hasAuthorship W1986720797A5066168948 @default.
• W1986720797 hasAuthorship W1986720797A5079125985 @default.

• next