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- W1986734565 abstract "Calpastatin, an endogeneous inhibitor protein acting on calpain (Ca(2+)-dependent cysteine proteinase), is widely distributed in animal tissues and cells. Two different expression systems, baculovirus-infected Spodoptera frugiperda (Sf9) insect cells and Escherichia coli, were used for overexpression of the human calpastatin tagged with N-terminal hexahistidine peptide. Recombinant calpastatin was purified to homogeneity by nickel ion affinity chromatography and gel filtration separation. Purified recombinant proteins from both systems have similar inhibitory activity for calpain." @default.
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- W1986734565 date "1998-01-01" @default.
- W1986734565 modified "2023-09-26" @default.
- W1986734565 title "Expression of Biologically Active Human Calpastatin in Baculovirus-infected Insect Cells and in<i>Escherichia coli</i>" @default.
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- W1986734565 doi "https://doi.org/10.1271/bbb.62.136" @default.
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