FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1986770169> ?p ?o ?g. }

• W1986770169 endingPage "7125" @default.
• W1986770169 startingPage "7119" @default.
• W1986770169 abstract "The bifunctional wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase (WS/DGAT) is the key enzyme in storage lipid accumulation in the gram-negative bacterium Acinetobacter calcoaceticus ADP1, mediating wax ester, and to a lesser extent, triacylglycerol (TAG) biosynthesis. Saccharomyces cerevisiae accumulates TAGs and steryl esters as storage lipids. Four genes encoding a DGAT (Dga1p), a phospholipid:diacylglycerol acyltransferase (Lro1p) and two acyl-coenzyme A:sterol acyltransferases (ASATs) (Are1p and Are2p) are involved in the final esterification steps in TAG and steryl ester biosynthesis in this yeast. In the quadruple mutant strain S. cerevisiae H1246, the disruption of DGA1, LRO1, ARE1, and ARE2 leads to an inability to synthesize storage lipids. Heterologous expression of WS/DGAT from A. calcoaceticus ADP1 in S. cerevisiae H1246 restored TAG but not steryl ester biosynthesis, although high levels of ASAT activity could be demonstrated for WS/DGAT expressed in Escherichia coli XL1-Blue in radiometric in vitro assays with cholesterol and ergosterol as substrates. In addition to TAG synthesis, heterologous expression of WS/DGAT in S. cerevisiae H1246 resulted also in the accumulation of fatty acid ethyl esters as well as fatty acid isoamyl esters. In vitro studies confirmed that WS/DGAT is capable of utilizing a broad range of alcohols as substrates comprising long-chain fatty alcohols like hexadecanol as well as short-chain alcohols like ethanol or isoamyl alcohol. This study demonstrated the highly unspecific acyltransferase activity of WS/DGAT from A. calcoaceticus ADP1, indicating the broad biocatalytic potential of this enzyme for biotechnological production of a large variety of lipids in vivo in prokaryotic as well as eukaryotic expression hosts." @default.
• W1986770169 created "2016-06-24" @default.
• W1986770169 creator A5002814642 @default.
• W1986770169 creator A5058839240 @default.
• W1986770169 creator A5076768521 @default.
• W1986770169 date "2004-12-01" @default.
• W1986770169 modified "2023-10-14" @default.
• W1986770169 title "Synthesis of Novel Lipids in <i>Saccharomyces cerevisiae</i> by Heterologous Expression of an Unspecific Bacterial Acyltransferase" @default.
• W1986770169 cites W1559748645 @default.
• W1986770169 cites W1570952215 @default.
• W1986770169 cites W1896630930 @default.
• W1986770169 cites W1973782344 @default.
• W1986770169 cites W1983091826 @default.
• W1986770169 cites W1987933395 @default.
• W1986770169 cites W1996953184 @default.
• W1986770169 cites W1999899846 @default.
• W1986770169 cites W2007396399 @default.
• W1986770169 cites W2011805571 @default.
• W1986770169 cites W2018917785 @default.
• W1986770169 cites W2023555443 @default.
• W1986770169 cites W2026487666 @default.
• W1986770169 cites W2028123717 @default.
• W1986770169 cites W2031805875 @default.
• W1986770169 cites W2039481955 @default.
• W1986770169 cites W2043237666 @default.
• W1986770169 cites W2045348184 @default.
• W1986770169 cites W2049661143 @default.
• W1986770169 cites W2057480992 @default.
• W1986770169 cites W2061927321 @default.
• W1986770169 cites W2065967474 @default.
• W1986770169 cites W2066443005 @default.
• W1986770169 cites W2067310352 @default.
• W1986770169 cites W2077068031 @default.
• W1986770169 cites W2087805964 @default.
• W1986770169 cites W2089169103 @default.
• W1986770169 cites W2094758309 @default.
• W1986770169 cites W2096749734 @default.
• W1986770169 cites W2102949501 @default.
• W1986770169 cites W2103016670 @default.
• W1986770169 cites W2111771826 @default.
• W1986770169 cites W2122772014 @default.
• W1986770169 cites W2155744724 @default.
• W1986770169 cites W2161064715 @default.
• W1986770169 cites W4211151192 @default.
• W1986770169 doi "https://doi.org/10.1128/aem.70.12.7119-7125.2004" @default.
• W1986770169 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/535189" @default.
• W1986770169 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15574908" @default.
• W1986770169 hasPublicationYear "2004" @default.
• W1986770169 type Work @default.
• W1986770169 sameAs 1986770169 @default.
• W1986770169 citedByCount "119" @default.
• W1986770169 countsByYear W19867701692012 @default.
• W1986770169 countsByYear W19867701692013 @default.
• W1986770169 countsByYear W19867701692014 @default.
• W1986770169 countsByYear W19867701692015 @default.
• W1986770169 countsByYear W19867701692016 @default.
• W1986770169 countsByYear W19867701692017 @default.
• W1986770169 countsByYear W19867701692018 @default.
• W1986770169 countsByYear W19867701692019 @default.
• W1986770169 countsByYear W19867701692020 @default.
• W1986770169 countsByYear W19867701692021 @default.
• W1986770169 countsByYear W19867701692022 @default.
• W1986770169 countsByYear W19867701692023 @default.
• W1986770169 crossrefType "journal-article" @default.
• W1986770169 hasAuthorship W1986770169A5002814642 @default.
• W1986770169 hasAuthorship W1986770169A5058839240 @default.
• W1986770169 hasAuthorship W1986770169A5076768521 @default.
• W1986770169 hasBestOaLocation W19867701691 @default.
• W1986770169 hasConcept C104317684 @default.
• W1986770169 hasConcept C108305142 @default.
• W1986770169 hasConcept C134651460 @default.
• W1986770169 hasConcept C181199279 @default.
• W1986770169 hasConcept C185592680 @default.
• W1986770169 hasConcept C2775970874 @default.
• W1986770169 hasConcept C2776288157 @default.
• W1986770169 hasConcept C2776452521 @default.
• W1986770169 hasConcept C2777576037 @default.
• W1986770169 hasConcept C2779123261 @default.
• W1986770169 hasConcept C2779222958 @default.
• W1986770169 hasConcept C2781066024 @default.
• W1986770169 hasConcept C2910079589 @default.
• W1986770169 hasConcept C40767141 @default.
• W1986770169 hasConcept C543025807 @default.
• W1986770169 hasConcept C553450214 @default.
• W1986770169 hasConcept C55493867 @default.
• W1986770169 hasConcept C86803240 @default.
• W1986770169 hasConceptScore W1986770169C104317684 @default.
• W1986770169 hasConceptScore W1986770169C108305142 @default.
• W1986770169 hasConceptScore W1986770169C134651460 @default.
• W1986770169 hasConceptScore W1986770169C181199279 @default.
• W1986770169 hasConceptScore W1986770169C185592680 @default.
• W1986770169 hasConceptScore W1986770169C2775970874 @default.
• W1986770169 hasConceptScore W1986770169C2776288157 @default.
• W1986770169 hasConceptScore W1986770169C2776452521 @default.
• W1986770169 hasConceptScore W1986770169C2777576037 @default.
• W1986770169 hasConceptScore W1986770169C2779123261 @default.
• W1986770169 hasConceptScore W1986770169C2779222958 @default.
• W1986770169 hasConceptScore W1986770169C2781066024 @default.

• next