FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1986875563> ?p ?o ?g. }

• W1986875563 endingPage "6398" @default.
• W1986875563 startingPage "6389" @default.
• W1986875563 abstract "Gonadotrope cells are primarily regulated by GnRH but are also targets of the pituitary adenylyl cyclase-activating polypeptide (PACAP). Although it has been reported that reciprocal interactions between both neuropeptides contribute to regulation of gonadotrope function, the underlying mechanisms remain poorly understood. In this study, we reevaluated PACAP coupling to the cAMP pathway in LβT2 gonadotrope cells and analyzed GnRH effect on PACAP signaling. We established that PACAP38 markedly increases intracellular cAMP levels (EC50 of 4.7 ± 1.3 nm) through the PACAP type 1 receptor (PAC1-R), as evidenced by pharmacological and RT-PCR studies. Interestingly, although GnRH couples to cAMP pathway in LβT2 cells, the effects of both neuropeptides were not synergistic. Instead, the GnRH agonist (GnRHa) triptorelin rapidly and strongly inhibited (70% inhibition as early as 5 min) PACAP38-induced cAMP production. Inhibition was calcium independent, mimicked by the phorbol ester phorbol 12-myristate 13-acetate, and blocked by the protein kinase C (PKC) inhibitor bisindoylmaleimide, indicating that GnRHa inhibitory action relies on PKC. Selective down-regulation of both conventional and novel PKC prevented a GnRHa effect, whereas pharmacological inhibition of conventional PKC only was ineffective, strongly suggesting the involvement of novel PKC isoforms. GnRHa did not inhibit forskolin- or cholera toxin-stimulated cAMP accumulation, suggesting that PAC1-R is the predominant target of GnRH. Accordingly, we demonstrated for the first time that GnRH increases PAC1-R phosphorylation through PKC, providing a potential molecular mechanism which may account for GnRH inhibitory effect." @default.
• W1986875563 created "2016-06-24" @default.
• W1986875563 creator A5020328262 @default.
• W1986875563 creator A5024175389 @default.
• W1986875563 creator A5031845625 @default.
• W1986875563 creator A5058123444 @default.
• W1986875563 creator A5066749218 @default.
• W1986875563 creator A5069871672 @default.
• W1986875563 creator A5087998244 @default.
• W1986875563 date "2008-08-28" @default.
• W1986875563 modified "2023-10-18" @default.
• W1986875563 title "Gonadotropin-Releasing Hormone Inhibits Pituitary Adenylyl Cyclase-Activating Polypeptide Coupling to 3′,5′-Cyclic Adenosine-5′-Monophosphate Pathway in LβT2 Gonadotrope Cells through Novel Protein Kinase C Isoforms and Phosphorylation of Pituitary Adenylyl Cyclase-Activating Polypeptide Type I Receptor" @default.
• W1986875563 cites W1969288020 @default.
• W1986875563 cites W1971274261 @default.
• W1986875563 cites W1978319346 @default.
• W1986875563 cites W1981423842 @default.
• W1986875563 cites W1982565891 @default.
• W1986875563 cites W1986072099 @default.
• W1986875563 cites W1988097469 @default.
• W1986875563 cites W1993132699 @default.
• W1986875563 cites W1995942931 @default.
• W1986875563 cites W1997262454 @default.
• W1986875563 cites W1998132966 @default.
• W1986875563 cites W1998286599 @default.
• W1986875563 cites W2004142984 @default.
• W1986875563 cites W2006675789 @default.
• W1986875563 cites W2007210832 @default.
• W1986875563 cites W2009038754 @default.
• W1986875563 cites W2016295509 @default.
• W1986875563 cites W2016346332 @default.
• W1986875563 cites W2025414496 @default.
• W1986875563 cites W2026209290 @default.
• W1986875563 cites W2030821853 @default.
• W1986875563 cites W2032222143 @default.
• W1986875563 cites W2035507441 @default.
• W1986875563 cites W2039642408 @default.
• W1986875563 cites W2041797033 @default.
• W1986875563 cites W2041857039 @default.
• W1986875563 cites W2047428822 @default.
• W1986875563 cites W2051873694 @default.
• W1986875563 cites W2052146020 @default.
• W1986875563 cites W2054201526 @default.
• W1986875563 cites W2063535351 @default.
• W1986875563 cites W2074259011 @default.
• W1986875563 cites W2074580642 @default.
• W1986875563 cites W2080932789 @default.
• W1986875563 cites W2116269982 @default.
• W1986875563 cites W2116931151 @default.
• W1986875563 cites W2129486801 @default.
• W1986875563 cites W2149121207 @default.
• W1986875563 cites W2150769574 @default.
• W1986875563 cites W2156837840 @default.
• W1986875563 cites W2164842539 @default.
• W1986875563 cites W2397861637 @default.
• W1986875563 doi "https://doi.org/10.1210/en.2008-0504" @default.
• W1986875563 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18755795" @default.
• W1986875563 hasPublicationYear "2008" @default.
• W1986875563 type Work @default.
• W1986875563 sameAs 1986875563 @default.
• W1986875563 citedByCount "21" @default.
• W1986875563 countsByYear W19868755632012 @default.
• W1986875563 countsByYear W19868755632013 @default.
• W1986875563 countsByYear W19868755632014 @default.
• W1986875563 countsByYear W19868755632016 @default.
• W1986875563 countsByYear W19868755632018 @default.
• W1986875563 countsByYear W19868755632019 @default.
• W1986875563 countsByYear W19868755632020 @default.
• W1986875563 crossrefType "journal-article" @default.
• W1986875563 hasAuthorship W1986875563A5020328262 @default.
• W1986875563 hasAuthorship W1986875563A5024175389 @default.
• W1986875563 hasAuthorship W1986875563A5031845625 @default.
• W1986875563 hasAuthorship W1986875563A5058123444 @default.
• W1986875563 hasAuthorship W1986875563A5066749218 @default.
• W1986875563 hasAuthorship W1986875563A5069871672 @default.
• W1986875563 hasAuthorship W1986875563A5087998244 @default.
• W1986875563 hasBestOaLocation W19868755631 @default.
• W1986875563 hasConcept C11960822 @default.
• W1986875563 hasConcept C126322002 @default.
• W1986875563 hasConcept C134018914 @default.
• W1986875563 hasConcept C170493617 @default.
• W1986875563 hasConcept C185592680 @default.
• W1986875563 hasConcept C195794163 @default.
• W1986875563 hasConcept C197012351 @default.
• W1986875563 hasConcept C24998067 @default.
• W1986875563 hasConcept C2776668983 @default.
• W1986875563 hasConcept C2776991684 @default.
• W1986875563 hasConcept C2777006276 @default.
• W1986875563 hasConcept C2777658017 @default.
• W1986875563 hasConcept C2778575703 @default.
• W1986875563 hasConcept C2778894405 @default.
• W1986875563 hasConcept C2778938600 @default.
• W1986875563 hasConcept C2779178603 @default.
• W1986875563 hasConcept C2779276759 @default.
• W1986875563 hasConcept C2780043322 @default.
• W1986875563 hasConcept C62478195 @default.
• W1986875563 hasConcept C71315377 @default.
• W1986875563 hasConcept C71924100 @default.
• W1986875563 hasConcept C86803240 @default.

• next