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- W1986953410 abstract "We have examined individual GATC sites throughout the E. coli genome for their kinetics of remethylation by dam methyltransferase following the passage of the chromosomal replication fork. We present evidence for three major conclusions: that oriC is a single function unit that is specifically sequestered from dam methyltransferase for a significant period of time and then released; that the dnaA promoter region is subject to sequestration analogous to that observed at oriC and thus that hemimethylation-dependent sequestration is a general phenomenon; and that each round of replication initiation triggers a transient, temporally coordinate block in both reinitiation at oriC and expression of the dnaA gene. These and other observations are all consistent with the notion that hemimethylation in these two regions acts coordinately to ensure that every origin undergoes initiation once and only once per cell cycle; other possible roles for sequestration at dnaA are also considered." @default.
- W1986953410 created "2016-06-24" @default.
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- W1986953410 date "1968-01-01" @default.
- W1986953410 modified "2023-09-27" @default.
- W1986953410 title "A comparison of [13C]glucose-15NH4Cl with 5-bromouracil as density label in two experiments on sequential DNA replication in Escherichia coli 15T−" @default.
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- W1986953410 doi "https://doi.org/10.1016/0022-2836(68)90065-x" @default.
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