Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987013095> ?p ?o ?g. }
- W1987013095 endingPage "6026" @default.
- W1987013095 startingPage "6016" @default.
- W1987013095 abstract "Sphingolipids (SLs) play critical roles in eukaryotic cells in the formation of lipid rafts, membrane trafficking, and signal transduction. Here we created a SL null mutant in the protozoan parasite Leishmania major through targeted deletion of the key de novo biosynthetic enzyme serine palmitoyltransferase subunit 2 (SPT2). Although SLs are typically essential, spt2- Leishmania were viable, yet were completely deficient in de novo sphingolipid synthesis, and lacked inositol phosphorylceramides and other SLs. Remarkably, spt2- parasites maintained 'lipid rafts' as defined by Triton X-100 detergent resistant membrane formation. Upon entry to stationary phase spt2- failed to differentiate to infective metacyclic parasites and died instead. Death occurred not by apoptosis or changes in metacyclic gene expression, but from catastrophic problems leading to accumulation of small vesicles characteristic of the multivesicular body/multivesicular tubule network. Stage specificity may reflect changes in membrane structure as well as elevated demands in vesicular trafficking required for parasite remodeling during differentiation. We suggest that SL-deficient Leishmania provide a useful biological setting for tests of essential SL enzymes in other organisms where SL perturbation is lethal." @default.
- W1987013095 created "2016-06-24" @default.
- W1987013095 creator A5003850660 @default.
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- W1987013095 creator A5025482937 @default.
- W1987013095 creator A5034355519 @default.
- W1987013095 creator A5038207517 @default.
- W1987013095 date "2003-11-17" @default.
- W1987013095 modified "2023-10-18" @default.
- W1987013095 title "Sphingolipids are essential for differentiation but not growth in Leishmania" @default.
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