FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987014746> ?p ?o ?g. }

• W1987014746 endingPage "758" @default.
• W1987014746 startingPage "745" @default.
• W1987014746 abstract "Using the techniques of high performance liquid chromatography (HPLC), gel electrophoresis in the presence of sodium dodecyl sulfate (SDS), and immunoblotting, we have analyzed the age-related changes in soluble crystallins of the human lens. A 3 mm core along the optical axis of each lens was frozen-sectioned and the sections were biochemically analyzed for distribution and quantity of the various soluble protein species. Both cortical and nuclear samples show a monotonic decrease in the concentration of the 19 000 and 21 000 MW proteins with age. We find that these proteins behave anomalously on SDS-polyacrylamide gels, running near the top of the gel when the samples are not boiled before loading; this permitted us to observe the gradual, age-related loss of these bands from the gels of both nuclear and cortical samples. The high molecular weight, or TSK-3000 void volume, fraction (greater than 350,000) of the cortex contained alpha crystallin at all ages. However, in the nucleus, while this fraction is primarily composed of alpha crystallin early in life (i.e. before 15 years of age), there is a gradual incorporation of other crystallins into the void volume. This change in the composition of the high-molecular-weight, soluble protein fraction is reflected in: a change in the subunit mobility on SDS-polyacrylamide gels; reactivity of the fraction to crystallin antibodies, i.e. in the young nucleus there is reactivity to anti-alpha crystallin only, with a gradual increase in reactivity to anti-beta and anti-gamma crystallins. The void volume fraction of the nucleus persists as a major component of the soluble protein pool until 42-44 years of age, at which time the proportion of the total soluble protein represented by this void volume fraction decreases precipitously. These changes in the soluble protein profile are discussed in terms of their potential influence on the functioning of the lens." @default.
• W1987014746 created "2016-06-24" @default.
• W1987014746 creator A5000208164 @default.
• W1987014746 creator A5031937602 @default.
• W1987014746 creator A5051428701 @default.
• W1987014746 creator A5066854694 @default.
• W1987014746 date "1985-12-01" @default.
• W1987014746 modified "2023-09-26" @default.
• W1987014746 title "Spatial and temporal mapping of the age-related changes in human lens crystallins" @default.
• W1987014746 cites W1539888035 @default.
• W1987014746 cites W1993783478 @default.
• W1987014746 cites W2002981207 @default.
• W1987014746 cites W2012631379 @default.
• W1987014746 cites W2012697282 @default.
• W1987014746 cites W2024120301 @default.
• W1987014746 cites W2028907200 @default.
• W1987014746 cites W2030584905 @default.
• W1987014746 cites W2033585034 @default.
• W1987014746 cites W2041340703 @default.
• W1987014746 cites W2068882937 @default.
• W1987014746 cites W2073235249 @default.
• W1987014746 cites W2079409268 @default.
• W1987014746 cites W2079516125 @default.
• W1987014746 cites W2081696505 @default.
• W1987014746 cites W2094766444 @default.
• W1987014746 cites W2173237764 @default.
• W1987014746 doi "https://doi.org/10.1016/0014-4835(85)90183-6" @default.
• W1987014746 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3830737" @default.
• W1987014746 hasPublicationYear "1985" @default.
• W1987014746 type Work @default.
• W1987014746 sameAs 1987014746 @default.
• W1987014746 citedByCount "92" @default.
• W1987014746 countsByYear W19870147462012 @default.
• W1987014746 countsByYear W19870147462013 @default.
• W1987014746 countsByYear W19870147462014 @default.
• W1987014746 countsByYear W19870147462015 @default.
• W1987014746 countsByYear W19870147462016 @default.
• W1987014746 countsByYear W19870147462017 @default.
• W1987014746 countsByYear W19870147462018 @default.
• W1987014746 countsByYear W19870147462019 @default.
• W1987014746 countsByYear W19870147462020 @default.
• W1987014746 countsByYear W19870147462021 @default.
• W1987014746 countsByYear W19870147462022 @default.
• W1987014746 crossrefType "journal-article" @default.
• W1987014746 hasAuthorship W1987014746A5000208164 @default.
• W1987014746 hasAuthorship W1987014746A5031937602 @default.
• W1987014746 hasAuthorship W1987014746A5051428701 @default.
• W1987014746 hasAuthorship W1987014746A5066854694 @default.
• W1987014746 hasConcept C101660505 @default.
• W1987014746 hasConcept C116084860 @default.
• W1987014746 hasConcept C12554922 @default.
• W1987014746 hasConcept C142724271 @default.
• W1987014746 hasConcept C179998833 @default.
• W1987014746 hasConcept C181199279 @default.
• W1987014746 hasConcept C185592680 @default.
• W1987014746 hasConcept C204787440 @default.
• W1987014746 hasConcept C2776910235 @default.
• W1987014746 hasConcept C2778913249 @default.
• W1987014746 hasConcept C2780723820 @default.
• W1987014746 hasConcept C43617362 @default.
• W1987014746 hasConcept C55493867 @default.
• W1987014746 hasConcept C71924100 @default.
• W1987014746 hasConcept C7263354 @default.
• W1987014746 hasConcept C86803240 @default.
• W1987014746 hasConcept C95444343 @default.
• W1987014746 hasConceptScore W1987014746C101660505 @default.
• W1987014746 hasConceptScore W1987014746C116084860 @default.
• W1987014746 hasConceptScore W1987014746C12554922 @default.
• W1987014746 hasConceptScore W1987014746C142724271 @default.
• W1987014746 hasConceptScore W1987014746C179998833 @default.
• W1987014746 hasConceptScore W1987014746C181199279 @default.
• W1987014746 hasConceptScore W1987014746C185592680 @default.
• W1987014746 hasConceptScore W1987014746C204787440 @default.
• W1987014746 hasConceptScore W1987014746C2776910235 @default.
• W1987014746 hasConceptScore W1987014746C2778913249 @default.
• W1987014746 hasConceptScore W1987014746C2780723820 @default.
• W1987014746 hasConceptScore W1987014746C43617362 @default.
• W1987014746 hasConceptScore W1987014746C55493867 @default.
• W1987014746 hasConceptScore W1987014746C71924100 @default.
• W1987014746 hasConceptScore W1987014746C7263354 @default.
• W1987014746 hasConceptScore W1987014746C86803240 @default.
• W1987014746 hasConceptScore W1987014746C95444343 @default.
• W1987014746 hasIssue "6" @default.
• W1987014746 hasLocation W19870147461 @default.
• W1987014746 hasLocation W19870147462 @default.
• W1987014746 hasOpenAccess W1987014746 @default.
• W1987014746 hasPrimaryLocation W19870147461 @default.
• W1987014746 hasRelatedWork W1967477789 @default.
• W1987014746 hasRelatedWork W1970384624 @default.
• W1987014746 hasRelatedWork W1990549102 @default.
• W1987014746 hasRelatedWork W2011707458 @default.
• W1987014746 hasRelatedWork W2014242787 @default.
• W1987014746 hasRelatedWork W2035973499 @default.
• W1987014746 hasRelatedWork W2052406471 @default.
• W1987014746 hasRelatedWork W2060124553 @default.
• W1987014746 hasRelatedWork W2085640369 @default.
• W1987014746 hasRelatedWork W2168294295 @default.
• W1987014746 hasVolume "41" @default.

• next