Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987033090> ?p ?o ?g. }
Showing items 1 to 98 of
98
with 100 items per page.
- W1987033090 endingPage "166" @default.
- W1987033090 startingPage "162" @default.
- W1987033090 abstract "Recombinant proteins are of great commercial and scientific interest. However, most current production methods using mammalian cells involve the time- and labor-intensive step of creating stable cell lines. Although production methods based on transient gene expression could offer a significant improvement, transient transfection is currently still limited by low titers and low specific productivity compared to stable cell lines. To overcome these bottlenecks, we have explored the use of various growth factors to enhance specific productivity and titers in the context of transient gene expression. For that purpose, several growth factors were cloned and screened for their effect on transient gene expression in HEK293E and CHO-DG44 cells. In particular, acidic fibroblast growth factor (aFGF) was able to increase specific productivity by 60% and recombinant protein titers by 80% in HEK293E cells, while FGF9 increased titers by 250% in CHO-DG44 cells." @default.
- W1987033090 created "2016-06-24" @default.
- W1987033090 creator A5006066527 @default.
- W1987033090 creator A5033465736 @default.
- W1987033090 creator A5047674730 @default.
- W1987033090 creator A5068553938 @default.
- W1987033090 creator A5087001883 @default.
- W1987033090 date "2008-10-01" @default.
- W1987033090 modified "2023-10-17" @default.
- W1987033090 title "Coexpression of acidic fibroblast growth factor enhances specific productivity and antibody titers in transiently transfected HEK293 cells" @default.
- W1987033090 cites W1963910405 @default.
- W1987033090 cites W2031485466 @default.
- W1987033090 cites W2035671450 @default.
- W1987033090 cites W2074487444 @default.
- W1987033090 cites W2096868507 @default.
- W1987033090 cites W2099934038 @default.
- W1987033090 cites W2117471612 @default.
- W1987033090 cites W2118540273 @default.
- W1987033090 cites W2124203273 @default.
- W1987033090 cites W2130314959 @default.
- W1987033090 cites W2136476953 @default.
- W1987033090 cites W2147816873 @default.
- W1987033090 cites W2154192760 @default.
- W1987033090 cites W2164953211 @default.
- W1987033090 doi "https://doi.org/10.1016/j.nbt.2008.08.007" @default.
- W1987033090 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18804191" @default.
- W1987033090 hasPublicationYear "2008" @default.
- W1987033090 type Work @default.
- W1987033090 sameAs 1987033090 @default.
- W1987033090 citedByCount "21" @default.
- W1987033090 countsByYear W19870330902012 @default.
- W1987033090 countsByYear W19870330902013 @default.
- W1987033090 countsByYear W19870330902014 @default.
- W1987033090 countsByYear W19870330902015 @default.
- W1987033090 countsByYear W19870330902018 @default.
- W1987033090 countsByYear W19870330902019 @default.
- W1987033090 countsByYear W19870330902020 @default.
- W1987033090 countsByYear W19870330902023 @default.
- W1987033090 crossrefType "journal-article" @default.
- W1987033090 hasAuthorship W1987033090A5006066527 @default.
- W1987033090 hasAuthorship W1987033090A5033465736 @default.
- W1987033090 hasAuthorship W1987033090A5047674730 @default.
- W1987033090 hasAuthorship W1987033090A5068553938 @default.
- W1987033090 hasAuthorship W1987033090A5087001883 @default.
- W1987033090 hasConcept C104317684 @default.
- W1987033090 hasConcept C151730666 @default.
- W1987033090 hasConcept C153911025 @default.
- W1987033090 hasConcept C159654299 @default.
- W1987033090 hasConcept C174510640 @default.
- W1987033090 hasConcept C175656101 @default.
- W1987033090 hasConcept C203014093 @default.
- W1987033090 hasConcept C2779343474 @default.
- W1987033090 hasConcept C32611913 @default.
- W1987033090 hasConcept C40767141 @default.
- W1987033090 hasConcept C54009773 @default.
- W1987033090 hasConcept C54355233 @default.
- W1987033090 hasConcept C55493867 @default.
- W1987033090 hasConcept C81885089 @default.
- W1987033090 hasConcept C86803240 @default.
- W1987033090 hasConcept C95444343 @default.
- W1987033090 hasConceptScore W1987033090C104317684 @default.
- W1987033090 hasConceptScore W1987033090C151730666 @default.
- W1987033090 hasConceptScore W1987033090C153911025 @default.
- W1987033090 hasConceptScore W1987033090C159654299 @default.
- W1987033090 hasConceptScore W1987033090C174510640 @default.
- W1987033090 hasConceptScore W1987033090C175656101 @default.
- W1987033090 hasConceptScore W1987033090C203014093 @default.
- W1987033090 hasConceptScore W1987033090C2779343474 @default.
- W1987033090 hasConceptScore W1987033090C32611913 @default.
- W1987033090 hasConceptScore W1987033090C40767141 @default.
- W1987033090 hasConceptScore W1987033090C54009773 @default.
- W1987033090 hasConceptScore W1987033090C54355233 @default.
- W1987033090 hasConceptScore W1987033090C55493867 @default.
- W1987033090 hasConceptScore W1987033090C81885089 @default.
- W1987033090 hasConceptScore W1987033090C86803240 @default.
- W1987033090 hasConceptScore W1987033090C95444343 @default.
- W1987033090 hasIssue "2-3" @default.
- W1987033090 hasLocation W19870330901 @default.
- W1987033090 hasLocation W19870330902 @default.
- W1987033090 hasOpenAccess W1987033090 @default.
- W1987033090 hasPrimaryLocation W19870330901 @default.
- W1987033090 hasRelatedWork W2053393868 @default.
- W1987033090 hasRelatedWork W2083274803 @default.
- W1987033090 hasRelatedWork W2349100023 @default.
- W1987033090 hasRelatedWork W2361843014 @default.
- W1987033090 hasRelatedWork W2369736488 @default.
- W1987033090 hasRelatedWork W2378791079 @default.
- W1987033090 hasRelatedWork W2378943006 @default.
- W1987033090 hasRelatedWork W2392112009 @default.
- W1987033090 hasRelatedWork W2401891876 @default.
- W1987033090 hasRelatedWork W1994721121 @default.
- W1987033090 hasVolume "25" @default.
- W1987033090 isParatext "false" @default.
- W1987033090 isRetracted "false" @default.
- W1987033090 magId "1987033090" @default.
- W1987033090 workType "article" @default.