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- W1987039967 abstract "Factors influencing adenovirus 5 recovery from seawater by virus concentrator methods were determined. A 19,000-fold concentration of 25 gal samples with a theoretical recovery efficiency of 90% was possible with input multiplicities of 1000 TCID50 units ml−1. Pre-treatment of orlon and cellulose acetate filters with beef extract or tween 80 solutions promoted adenovirus passage during sample clarification. Adenovirus adsorbed to textile and epoxy fiberglass filters at acid pH. Adsorption to textile filters was enhanced by 0–05 m MgCl2. No salt enhancement was necessary for adsorption to epoxy fiberglass filters. Adenovirus was recovered from adsorbent filters following elution with 3% beef extract solution adjusted to pH 9.0. Adenovirus was reconcentrated from beef extract eluates by aqueous polymer phase separation. Actual recovery of 106 PFU of adenovirus from 50 gal of a waste treatment plant effluent was made with the modified virus concentrator procedure developed in the study." @default.
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- W1987039967 date "1975-04-01" @default.
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- W1987039967 title "Concentration of adenovirus from seawater" @default.
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- W1987039967 doi "https://doi.org/10.1016/0043-1354(75)90181-5" @default.
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