FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987065544> ?p ?o ?g. }

• W1987065544 endingPage "118" @default.
• W1987065544 startingPage "107" @default.
• W1987065544 abstract "Background: The mycotoxin ochratoxin A, an agent responsible for endemic Balkan nephropathy is known to trigger apoptosis and thus being toxic to several organs including the kidney. The mechanisms involved in ochratoxin A induced apoptosis include oxidative stress. Sequelae of ochratoxin intoxication include anemia. Similar to apoptosis of nucleated cells, erythrocytes may undergo suicidal cell death or eryptosis, which is characterized by cell shrinkage and cell membrane scrambling resulting in phosphatidylserine-exposure at the cell surface. Eryptosis could be triggered by Ca2+-entry through oxidant sensitive unspecificcation channels increasing cytosolic Ca2+ activity ([Ca2+]i). The Ca2+-sensitivity of cell membrane scrambling could be enhanced and eryptosis thus triggered by ceramide. The removal of suicidal erythrocytes may lead to anemia. Moreover, eryptotic erythrocytes could adhere to the vascular wall thus impeding microcirculation. The present study explored, whether ochratoxin A stimulates eryptosis. Methods: Fluo3-fluorescence was utilized to determine [Ca2+]i, forward scatter to estimate cell volume, annexin-V-binding to identify phosphatidylserine-exposing cells, fluorescent antibodies to detect ceramide formation and hemoglobin release to quantify hemolysis. Moreover, adhesion to human vascular endothelial cells (HUVEC) was determined utilizing a flow chamber. Results: A 48 h exposure to ochratoxin A was followed by significant increase of Fluo3-fluorescencei (≥ 2.5 µM), increase of ceramide abundance (10 µM), decrease of forward scatter (≥ 5 µM) and increase of annexin-V-binding (≥ 2.5 µM). Ochratoxin A exposure slightly but significantly enhanced hemolysis (10 µM). Ochratoxin (10 µM) enhanced erythrocyte adhesion to HUVEC. Removal of extracellular Ca2+ significantly blunted, but did not abrogate ochratoxin A-induced annexin V binding. Conclusions: Ochratoxin A triggers suicidal erythrocyte death or eryptosis, an effect partially but not fully due to stimulation of Ca2+-entry." @default.
• W1987065544 created "2016-06-24" @default.
• W1987065544 creator A5016914954 @default.
• W1987065544 creator A5027974474 @default.
• W1987065544 creator A5031007425 @default.
• W1987065544 creator A5051010945 @default.
• W1987065544 creator A5057733439 @default.
• W1987065544 creator A5084208566 @default.
• W1987065544 date "2012-01-01" @default.
• W1987065544 modified "2023-09-27" @default.
• W1987065544 title "Enhanced Apoptotic Death of Erythrocytes Induced by the Mycotoxin Ochratoxin A" @default.
• W1987065544 cites W13114315 @default.
• W1987065544 cites W1608710441 @default.
• W1987065544 cites W1963640549 @default.
• W1987065544 cites W1970513533 @default.
• W1987065544 cites W1971725708 @default.
• W1987065544 cites W1972660643 @default.
• W1987065544 cites W1973225321 @default.
• W1987065544 cites W1976466808 @default.
• W1987065544 cites W1980359334 @default.
• W1987065544 cites W1980548122 @default.
• W1987065544 cites W1983943582 @default.
• W1987065544 cites W1984504487 @default.
• W1987065544 cites W1985451889 @default.
• W1987065544 cites W1990356838 @default.
• W1987065544 cites W1991516089 @default.
• W1987065544 cites W1992180600 @default.
• W1987065544 cites W1993657855 @default.
• W1987065544 cites W1995487652 @default.
• W1987065544 cites W1996497038 @default.
• W1987065544 cites W1996741798 @default.
• W1987065544 cites W2003264522 @default.
• W1987065544 cites W2003679225 @default.
• W1987065544 cites W2004535701 @default.
• W1987065544 cites W2005794751 @default.
• W1987065544 cites W2006082963 @default.
• W1987065544 cites W2008603261 @default.
• W1987065544 cites W2018369904 @default.
• W1987065544 cites W2019005050 @default.
• W1987065544 cites W2019471670 @default.
• W1987065544 cites W2021722692 @default.
• W1987065544 cites W2025567292 @default.
• W1987065544 cites W2026598436 @default.
• W1987065544 cites W2026962502 @default.
• W1987065544 cites W2028325052 @default.
• W1987065544 cites W2030755334 @default.
• W1987065544 cites W2031903488 @default.
• W1987065544 cites W2032617178 @default.
• W1987065544 cites W2036961219 @default.
• W1987065544 cites W2037211493 @default.
• W1987065544 cites W2038070289 @default.
• W1987065544 cites W2038819783 @default.
• W1987065544 cites W2039616271 @default.
• W1987065544 cites W2041630868 @default.
• W1987065544 cites W2045148265 @default.
• W1987065544 cites W2047558995 @default.
• W1987065544 cites W2047761142 @default.
• W1987065544 cites W2050122986 @default.
• W1987065544 cites W2050571341 @default.
• W1987065544 cites W2051520080 @default.
• W1987065544 cites W2052123857 @default.
• W1987065544 cites W2053147858 @default.
• W1987065544 cites W2053428816 @default.
• W1987065544 cites W2053871805 @default.
• W1987065544 cites W2054786210 @default.
• W1987065544 cites W2055409835 @default.
• W1987065544 cites W2056369070 @default.
• W1987065544 cites W2065196084 @default.
• W1987065544 cites W2070190033 @default.
• W1987065544 cites W2074728848 @default.
• W1987065544 cites W2078013016 @default.
• W1987065544 cites W2079242039 @default.
• W1987065544 cites W2080046576 @default.
• W1987065544 cites W2083138485 @default.
• W1987065544 cites W2084010249 @default.
• W1987065544 cites W2084508785 @default.
• W1987065544 cites W2087043434 @default.
• W1987065544 cites W2087308723 @default.
• W1987065544 cites W2088533769 @default.
• W1987065544 cites W2089281127 @default.
• W1987065544 cites W2089976554 @default.
• W1987065544 cites W2091633267 @default.
• W1987065544 cites W2094494114 @default.
• W1987065544 cites W2097098920 @default.
• W1987065544 cites W2098956886 @default.
• W1987065544 cites W2104740033 @default.
• W1987065544 cites W2111937773 @default.
• W1987065544 cites W2113053099 @default.
• W1987065544 cites W2115278949 @default.
• W1987065544 cites W2122881251 @default.
• W1987065544 cites W2128882073 @default.
• W1987065544 cites W2132153731 @default.
• W1987065544 cites W2135716490 @default.
• W1987065544 cites W2139953109 @default.
• W1987065544 cites W2142885312 @default.
• W1987065544 cites W2155183573 @default.
• W1987065544 cites W2155670471 @default.
• W1987065544 cites W2164509237 @default.

• next