FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987125968> ?p ?o ?g. }

• W1987125968 endingPage "973" @default.
• W1987125968 startingPage "969" @default.
• W1987125968 abstract "We found that semiconductor quantum dots (QDs) dramatically improved both product yield and specificity of PCR. The concentration of QDs is important for improving PCR amplification. In the presence of appropriate concentration of mercaptoacetic acid (MAA)-coated QDs, specificity and yield of PCR were enhanced. Also, strong nonspecific bands and weaker smeared bands were eliminated. At lower annealing temperatures (25–45 °C), addition of MAA-coated QDs into the PCR reagent produced specific PCR products without nonspecific sequence amplification. MAA alone did not improve PCR amplification. Streptavidin (SA) surface modified QDs with different size also effectively improved the specificity of PCR, demonstrating that the observed effect was not due to property of the QD surface but instead due to the QD itself. Bovine Serum Albumin (BSA) could relieve Taq polymerase from MAA-coated QDs in PCR by interaction with QDs and therefore imply that QDs improve specificity of PCR by interaction with Taq polymerase. These results demonstrate that QDs, added to reaction mixes at appropriate concentrations, can increase PCR yield and improve PCR specificity, even at low annealing temperatures. We assume that many different surface modified polymeric nanoparticles might have similar effects." @default.
• W1987125968 created "2016-06-24" @default.
• W1987125968 creator A5009049500 @default.
• W1987125968 creator A5024030923 @default.
• W1987125968 creator A5037112198 @default.
• W1987125968 creator A5037324922 @default.
• W1987125968 creator A5044852914 @default.
• W1987125968 creator A5071018088 @default.
• W1987125968 date "2009-08-01" @default.
• W1987125968 modified "2023-09-23" @default.
• W1987125968 title "Maximizing specificity and yield of PCR by the quantum dot itself rather than property of the quantum dot surface" @default.
• W1987125968 cites W1517480469 @default.
• W1987125968 cites W1586683291 @default.
• W1987125968 cites W1967238955 @default.
• W1987125968 cites W1968190169 @default.
• W1987125968 cites W1982950335 @default.
• W1987125968 cites W1984994174 @default.
• W1987125968 cites W1996596438 @default.
• W1987125968 cites W2011032800 @default.
• W1987125968 cites W2019026961 @default.
• W1987125968 cites W2021243296 @default.
• W1987125968 cites W2027177838 @default.
• W1987125968 cites W2029312076 @default.
• W1987125968 cites W2032118018 @default.
• W1987125968 cites W2039654089 @default.
• W1987125968 cites W2050717506 @default.
• W1987125968 cites W2053096433 @default.
• W1987125968 cites W2061445358 @default.
• W1987125968 cites W2062518418 @default.
• W1987125968 cites W2070789776 @default.
• W1987125968 cites W2078797793 @default.
• W1987125968 cites W2078856540 @default.
• W1987125968 cites W2080879716 @default.
• W1987125968 cites W2081422634 @default.
• W1987125968 cites W2082277951 @default.
• W1987125968 cites W2094437628 @default.
• W1987125968 cites W2097376269 @default.
• W1987125968 cites W2101648740 @default.
• W1987125968 cites W2115767337 @default.
• W1987125968 cites W2144175668 @default.
• W1987125968 cites W2144634347 @default.
• W1987125968 cites W2153436893 @default.
• W1987125968 cites W2154321074 @default.
• W1987125968 cites W2160580330 @default.
• W1987125968 doi "https://doi.org/10.1016/j.biochi.2009.04.020" @default.
• W1987125968 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19442702" @default.
• W1987125968 hasPublicationYear "2009" @default.
• W1987125968 type Work @default.
• W1987125968 sameAs 1987125968 @default.
• W1987125968 citedByCount "37" @default.
• W1987125968 countsByYear W19871259682012 @default.
• W1987125968 countsByYear W19871259682013 @default.
• W1987125968 countsByYear W19871259682014 @default.
• W1987125968 countsByYear W19871259682015 @default.
• W1987125968 countsByYear W19871259682016 @default.
• W1987125968 countsByYear W19871259682017 @default.
• W1987125968 countsByYear W19871259682018 @default.
• W1987125968 countsByYear W19871259682019 @default.
• W1987125968 countsByYear W19871259682020 @default.
• W1987125968 countsByYear W19871259682021 @default.
• W1987125968 countsByYear W19871259682022 @default.
• W1987125968 countsByYear W19871259682023 @default.
• W1987125968 crossrefType "journal-article" @default.
• W1987125968 hasAuthorship W1987125968A5009049500 @default.
• W1987125968 hasAuthorship W1987125968A5024030923 @default.
• W1987125968 hasAuthorship W1987125968A5037112198 @default.
• W1987125968 hasAuthorship W1987125968A5037324922 @default.
• W1987125968 hasAuthorship W1987125968A5044852914 @default.
• W1987125968 hasAuthorship W1987125968A5071018088 @default.
• W1987125968 hasConcept C104317684 @default.
• W1987125968 hasConcept C113196181 @default.
• W1987125968 hasConcept C121332964 @default.
• W1987125968 hasConcept C124657808 @default.
• W1987125968 hasConcept C12554922 @default.
• W1987125968 hasConcept C134121241 @default.
• W1987125968 hasConcept C147789679 @default.
• W1987125968 hasConcept C159985019 @default.
• W1987125968 hasConcept C171001562 @default.
• W1987125968 hasConcept C171250308 @default.
• W1987125968 hasConcept C185592680 @default.
• W1987125968 hasConcept C191897082 @default.
• W1987125968 hasConcept C192562407 @default.
• W1987125968 hasConcept C2776190903 @default.
• W1987125968 hasConcept C2777855556 @default.
• W1987125968 hasConcept C2778204606 @default.
• W1987125968 hasConcept C2779186596 @default.
• W1987125968 hasConcept C2781440490 @default.
• W1987125968 hasConcept C40875361 @default.
• W1987125968 hasConcept C43617362 @default.
• W1987125968 hasConcept C49105822 @default.
• W1987125968 hasConcept C552990157 @default.
• W1987125968 hasConcept C55493867 @default.
• W1987125968 hasConcept C62520636 @default.
• W1987125968 hasConcept C82381507 @default.
• W1987125968 hasConcept C86803240 @default.
• W1987125968 hasConcept C86807702 @default.
• W1987125968 hasConcept C91881484 @default.
• W1987125968 hasConceptScore W1987125968C104317684 @default.

• next