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- W1987133049 abstract "We developed a hue-based pH determination method to analyze digital images of samples in a 384-well plate after the addition of a universal pH indicator. The standard error of calibration for 69 pH standards was 0.078 pH units, and no sample gave an error greater than 0.23 units. We then used in-solution isoelectric focusing to determine the isoelectric point of Wnt3A protein in conditioned medium and after purification and applied the described method to assess the pH of these small-volume samples. End users may access our standard to assay the pH of their own samples with no additional calibration." @default.
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- W1987133049 date "2014-10-01" @default.
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- W1987133049 title "Quantitative pH assessment of small-volume samples using a universal pH indicator" @default.
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- W1987133049 doi "https://doi.org/10.1016/j.ab.2014.06.001" @default.
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