Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987133058> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W1987133058 endingPage "180" @default.
- W1987133058 startingPage "167" @default.
- W1987133058 abstract "Freezing and thawing a dialysed suspension of lysed chromaffin granules and sedimented membrane preparations resulted in redistribution of lipid phosphate and protein. By this treatment the high ratios of lipid phosphate/protein in the membrane fragments, isolated on sucrose density gradient from the dialysed suspensions and the sedimented membrane preparation, were reduced from 1.56 to 1.03 μmoles/mg and from 1.97 to 0.83 μmoles/mg, respectively. Multilamellar, liposomal structures could be isolated from the frozen and thawed membrane preparations and were found to sediment in the 0.4 M sucrose layer by density gradient centrigugation. This fraction was without morphological resemblance to the intact chromaffin granules or their membranes and was found to account for 53% of the lipid phosphate, 35% of chromogranin A, 21% of dopamine-β-hydroxylase activity and 12% of the protein of the total preparation. The specific activities of chromogranin A and dopamine-β-hydroxylase in the artificially formed liposomal structures closely resembled that of the solubilized protein and was significantly higher than in the lipid phosphate-depleted membrane fragments recovered in the 1.1 M sucrose layers. It is concluded that freezing and thawing as a means of purifying the isolated granule membranes lead not only to the solubilization of chromogranin A, but also to removal of dopamine-β-hydroxylase activity and lipid phosphate from the labile membrane fragments." @default.
- W1987133058 created "2016-06-24" @default.
- W1987133058 creator A5040308282 @default.
- W1987133058 date "1973-08-01" @default.
- W1987133058 modified "2023-10-17" @default.
- W1987133058 title "Biochemical studies of the chromaffin granule. III. Redistribution of lipid phosphate, dopamine-β-hydroxylase and chromogranin a after freezing and thawing of the isolated granule membranes" @default.
- W1987133058 cites W1528181398 @default.
- W1987133058 cites W1775749144 @default.
- W1987133058 cites W1967010896 @default.
- W1987133058 cites W1990710913 @default.
- W1987133058 cites W1996463863 @default.
- W1987133058 cites W1999654411 @default.
- W1987133058 cites W2003272223 @default.
- W1987133058 cites W2007534334 @default.
- W1987133058 cites W2016429077 @default.
- W1987133058 cites W2030670408 @default.
- W1987133058 cites W2049848042 @default.
- W1987133058 cites W2077926440 @default.
- W1987133058 cites W2087612678 @default.
- W1987133058 cites W2093169996 @default.
- W1987133058 cites W2102923474 @default.
- W1987133058 cites W2104571544 @default.
- W1987133058 cites W2107342271 @default.
- W1987133058 cites W2286011520 @default.
- W1987133058 cites W2337938824 @default.
- W1987133058 cites W2406280345 @default.
- W1987133058 cites W2408761274 @default.
- W1987133058 cites W2599186161 @default.
- W1987133058 doi "https://doi.org/10.1016/0005-2736(73)90111-9" @default.
- W1987133058 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4200828" @default.
- W1987133058 hasPublicationYear "1973" @default.
- W1987133058 type Work @default.
- W1987133058 sameAs 1987133058 @default.
- W1987133058 citedByCount "19" @default.
- W1987133058 crossrefType "journal-article" @default.
- W1987133058 hasAuthorship W1987133058A5040308282 @default.
- W1987133058 hasConcept C151730666 @default.
- W1987133058 hasConcept C185154212 @default.
- W1987133058 hasConcept C185592680 @default.
- W1987133058 hasConcept C203014093 @default.
- W1987133058 hasConcept C204232928 @default.
- W1987133058 hasConcept C2777132085 @default.
- W1987133058 hasConcept C2778597767 @default.
- W1987133058 hasConcept C39944091 @default.
- W1987133058 hasConcept C41625074 @default.
- W1987133058 hasConcept C43617362 @default.
- W1987133058 hasConcept C49760210 @default.
- W1987133058 hasConcept C55493867 @default.
- W1987133058 hasConcept C57409179 @default.
- W1987133058 hasConcept C77231918 @default.
- W1987133058 hasConcept C86803240 @default.
- W1987133058 hasConceptScore W1987133058C151730666 @default.
- W1987133058 hasConceptScore W1987133058C185154212 @default.
- W1987133058 hasConceptScore W1987133058C185592680 @default.
- W1987133058 hasConceptScore W1987133058C203014093 @default.
- W1987133058 hasConceptScore W1987133058C204232928 @default.
- W1987133058 hasConceptScore W1987133058C2777132085 @default.
- W1987133058 hasConceptScore W1987133058C2778597767 @default.
- W1987133058 hasConceptScore W1987133058C39944091 @default.
- W1987133058 hasConceptScore W1987133058C41625074 @default.
- W1987133058 hasConceptScore W1987133058C43617362 @default.
- W1987133058 hasConceptScore W1987133058C49760210 @default.
- W1987133058 hasConceptScore W1987133058C55493867 @default.
- W1987133058 hasConceptScore W1987133058C57409179 @default.
- W1987133058 hasConceptScore W1987133058C77231918 @default.
- W1987133058 hasConceptScore W1987133058C86803240 @default.
- W1987133058 hasIssue "2" @default.
- W1987133058 hasLocation W19871330581 @default.
- W1987133058 hasLocation W19871330582 @default.
- W1987133058 hasOpenAccess W1987133058 @default.
- W1987133058 hasPrimaryLocation W19871330581 @default.
- W1987133058 hasRelatedWork W124525801 @default.
- W1987133058 hasRelatedWork W2000737985 @default.
- W1987133058 hasRelatedWork W2005168949 @default.
- W1987133058 hasRelatedWork W2016682415 @default.
- W1987133058 hasRelatedWork W2043051289 @default.
- W1987133058 hasRelatedWork W2551968635 @default.
- W1987133058 hasRelatedWork W2604912652 @default.
- W1987133058 hasRelatedWork W3011024476 @default.
- W1987133058 hasRelatedWork W4283584018 @default.
- W1987133058 hasRelatedWork W181634280 @default.
- W1987133058 hasVolume "318" @default.
- W1987133058 isParatext "false" @default.
- W1987133058 isRetracted "false" @default.
- W1987133058 magId "1987133058" @default.
- W1987133058 workType "article" @default.