Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987158192> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W1987158192 endingPage "2496" @default.
- W1987158192 startingPage "2485" @default.
- W1987158192 abstract "Myosin IIIA is unique among myosin proteins in that it contains an N-terminal kinase domain capable of autophosphorylating sites on the motor domain. A construct of myosin IIIA lacking the kinase domain localizes more efficiently to the stereocilia tips and alters the morphology of the tips in inner ear hair cells. Therefore, we performed a kinetic analysis of myosin IIIA without the kinase domain (MIII ΔK) and compared these results with our reported analysis of myosin IIIA containing the kinase domain (MIII). The steady-state kinetic properties of MIII ΔK indicate that it has a 2-fold higher maximum actin-activated ATPase rate (kcat = 1.5 ± 0.1 s-1) and a 5-fold tighter actin affinity (KATPase = 6.0 ± 1.4 μM, and KActin = 1.4 ± 0.4 μM) compared to MIII. The rate of ATP binding to the motor domain is enhanced in MIII ΔK (K1k+2 ≈ 0.10 ± 0.01 μM-1·s-1) to a level similar to the rate of binding to MIII in the presence of actin. The rate of ATP hydrolysis in the absence of actin is slow and may be rate limiting. Actin-activated phosphate release is identical with and without the kinase domain. The transition between actomyosin.ADP states, which is rate limiting in MIII, is enhanced in MIII ΔK. MIII ΔK accumulates more efficiently at the tips of filopodia in HeLa cells. Our results suggest a model in which the activity and concentration of myosin IIIA localized to the tips of actin bundles mediates the morphology of the tips in sensory cells." @default.
- W1987158192 created "2016-06-24" @default.
- W1987158192 creator A5020062987 @default.
- W1987158192 creator A5025082181 @default.
- W1987158192 creator A5048632953 @default.
- W1987158192 creator A5051140095 @default.
- W1987158192 creator A5052510325 @default.
- W1987158192 creator A5053799479 @default.
- W1987158192 date "2008-01-30" @default.
- W1987158192 modified "2023-10-18" @default.
- W1987158192 title "The Kinase Domain Alters the Kinetic Properties of the Myosin IIIA Motor" @default.
- W1987158192 cites W1972858438 @default.
- W1987158192 cites W1980493113 @default.
- W1987158192 cites W1986912066 @default.
- W1987158192 cites W1991296405 @default.
- W1987158192 cites W2023296324 @default.
- W1987158192 cites W2030267692 @default.
- W1987158192 cites W2044597760 @default.
- W1987158192 cites W2071647584 @default.
- W1987158192 cites W2073841975 @default.
- W1987158192 cites W2088355600 @default.
- W1987158192 cites W2129954619 @default.
- W1987158192 cites W2130480967 @default.
- W1987158192 cites W2140994672 @default.
- W1987158192 cites W2156226827 @default.
- W1987158192 cites W2159503893 @default.
- W1987158192 cites W336760737 @default.
- W1987158192 doi "https://doi.org/10.1021/bi7021574" @default.
- W1987158192 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18229949" @default.
- W1987158192 hasPublicationYear "2008" @default.
- W1987158192 type Work @default.
- W1987158192 sameAs 1987158192 @default.
- W1987158192 citedByCount "28" @default.
- W1987158192 countsByYear W19871581922012 @default.
- W1987158192 countsByYear W19871581922013 @default.
- W1987158192 countsByYear W19871581922016 @default.
- W1987158192 countsByYear W19871581922017 @default.
- W1987158192 countsByYear W19871581922018 @default.
- W1987158192 countsByYear W19871581922020 @default.
- W1987158192 countsByYear W19871581922021 @default.
- W1987158192 countsByYear W19871581922022 @default.
- W1987158192 crossrefType "journal-article" @default.
- W1987158192 hasAuthorship W1987158192A5020062987 @default.
- W1987158192 hasAuthorship W1987158192A5025082181 @default.
- W1987158192 hasAuthorship W1987158192A5048632953 @default.
- W1987158192 hasAuthorship W1987158192A5051140095 @default.
- W1987158192 hasAuthorship W1987158192A5052510325 @default.
- W1987158192 hasAuthorship W1987158192A5053799479 @default.
- W1987158192 hasConcept C104317684 @default.
- W1987158192 hasConcept C12554922 @default.
- W1987158192 hasConcept C125705527 @default.
- W1987158192 hasConcept C141315368 @default.
- W1987158192 hasConcept C143065580 @default.
- W1987158192 hasConcept C181199279 @default.
- W1987158192 hasConcept C185592680 @default.
- W1987158192 hasConcept C23265538 @default.
- W1987158192 hasConcept C32619005 @default.
- W1987158192 hasConcept C55493867 @default.
- W1987158192 hasConcept C6997183 @default.
- W1987158192 hasConcept C8035138 @default.
- W1987158192 hasConcept C86803240 @default.
- W1987158192 hasConcept C90301020 @default.
- W1987158192 hasConcept C95444343 @default.
- W1987158192 hasConceptScore W1987158192C104317684 @default.
- W1987158192 hasConceptScore W1987158192C12554922 @default.
- W1987158192 hasConceptScore W1987158192C125705527 @default.
- W1987158192 hasConceptScore W1987158192C141315368 @default.
- W1987158192 hasConceptScore W1987158192C143065580 @default.
- W1987158192 hasConceptScore W1987158192C181199279 @default.
- W1987158192 hasConceptScore W1987158192C185592680 @default.
- W1987158192 hasConceptScore W1987158192C23265538 @default.
- W1987158192 hasConceptScore W1987158192C32619005 @default.
- W1987158192 hasConceptScore W1987158192C55493867 @default.
- W1987158192 hasConceptScore W1987158192C6997183 @default.
- W1987158192 hasConceptScore W1987158192C8035138 @default.
- W1987158192 hasConceptScore W1987158192C86803240 @default.
- W1987158192 hasConceptScore W1987158192C90301020 @default.
- W1987158192 hasConceptScore W1987158192C95444343 @default.
- W1987158192 hasIssue "8" @default.
- W1987158192 hasLocation W19871581921 @default.
- W1987158192 hasLocation W19871581922 @default.
- W1987158192 hasOpenAccess W1987158192 @default.
- W1987158192 hasPrimaryLocation W19871581921 @default.
- W1987158192 hasRelatedWork W1546918094 @default.
- W1987158192 hasRelatedWork W1968555063 @default.
- W1987158192 hasRelatedWork W2026315495 @default.
- W1987158192 hasRelatedWork W2043657521 @default.
- W1987158192 hasRelatedWork W2045079910 @default.
- W1987158192 hasRelatedWork W2057353555 @default.
- W1987158192 hasRelatedWork W2073413583 @default.
- W1987158192 hasRelatedWork W2615823300 @default.
- W1987158192 hasRelatedWork W2950414472 @default.
- W1987158192 hasRelatedWork W2969269896 @default.
- W1987158192 hasVolume "47" @default.
- W1987158192 isParatext "false" @default.
- W1987158192 isRetracted "false" @default.
- W1987158192 magId "1987158192" @default.
- W1987158192 workType "article" @default.