FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987163952> ?p ?o ?g. }

• W1987163952 endingPage "487" @default.
• W1987163952 startingPage "479" @default.
• W1987163952 abstract "Background Here we report on studies that probe whether the intracellular kinetics of plasmid DNA (pDNA) and cell surface glycosaminoglycans (GAGs) are modified during the cell cycle in a way that can be correlated with changes in gene transfer efficiency with poly(ethyleneimine) (PEI) and poly-L-lysine (PLL) polyplexes. Methods Synchronized D407 retinal cells were transfected with PEI and PLL polyplexes using a luciferase reporter. The free and/or loosely complexed nuclear pDNA was determined by real-time PCR, and compared with transgene expression, the rate of pinocytosis by FITC-dextran uptake and the content of cell surface GAGs. Results The amount of free and/or loosely complexed nuclear pDNA between cell cycle phases varied ∼4–20 times (G1 < S < G2/M). Both carriers delivered pDNA in a similar way into the nucleus (PLL vs. PEI ⩽ 3.5-fold), but PEI was ∼10–100 times more efficient in gene expression than PLL (G1 < G2/M < S). The rate of pinocytosis increased up to 70-fold from G1 to middle S phase. Cell surface heparan and chondroitin sulfate increased 50–80%, and hyaluronan decreased 50% when the cells went from G1 through S to G2/M. Conclusions The data obtained indicates that no single parameter (pinocytosis, cell surface GAGs, nuclear uptake) solely accounts for the differential pDNA uptake or expression during cell cycle, and that the main difference in PLL- and PEI-mediated transfections seems to be at the nuclear level. Copyright © 2007 John Wiley & Sons, Ltd." @default.
• W1987163952 created "2016-06-24" @default.
• W1987163952 creator A5014958903 @default.
• W1987163952 creator A5040073148 @default.
• W1987163952 creator A5065810893 @default.
• W1987163952 creator A5070879563 @default.
• W1987163952 creator A5088605616 @default.
• W1987163952 creator A5091424033 @default.
• W1987163952 date "2007-01-01" @default.
• W1987163952 modified "2023-10-11" @default.
• W1987163952 title "Polyplex-mediated gene transfer and cell cycle: effect of carrier on cellular uptake and intracellular kinetics, and significance of glycosaminoglycans" @default.
• W1987163952 cites W1575139878 @default.
• W1987163952 cites W1611708000 @default.
• W1987163952 cites W1920936907 @default.
• W1987163952 cites W1968098538 @default.
• W1987163952 cites W1968113316 @default.
• W1987163952 cites W1968496776 @default.
• W1987163952 cites W1975833492 @default.
• W1987163952 cites W1976030535 @default.
• W1987163952 cites W1976811878 @default.
• W1987163952 cites W1978035834 @default.
• W1987163952 cites W1993081501 @default.
• W1987163952 cites W2008835126 @default.
• W1987163952 cites W2010655741 @default.
• W1987163952 cites W2011138429 @default.
• W1987163952 cites W2013156289 @default.
• W1987163952 cites W2019394698 @default.
• W1987163952 cites W2020260050 @default.
• W1987163952 cites W2023842478 @default.
• W1987163952 cites W2031768903 @default.
• W1987163952 cites W2032689379 @default.
• W1987163952 cites W2034855849 @default.
• W1987163952 cites W2036747281 @default.
• W1987163952 cites W2041004522 @default.
• W1987163952 cites W2050891199 @default.
• W1987163952 cites W2054098399 @default.
• W1987163952 cites W2056111951 @default.
• W1987163952 cites W2060831721 @default.
• W1987163952 cites W2072064961 @default.
• W1987163952 cites W2074394573 @default.
• W1987163952 cites W2082204799 @default.
• W1987163952 cites W2089687270 @default.
• W1987163952 cites W2090211284 @default.
• W1987163952 cites W2091267783 @default.
• W1987163952 cites W2107929771 @default.
• W1987163952 cites W2127021351 @default.
• W1987163952 cites W2134344512 @default.
• W1987163952 cites W2142987628 @default.
• W1987163952 cites W2164473167 @default.
• W1987163952 cites W2325056821 @default.
• W1987163952 cites W2508485765 @default.
• W1987163952 doi "https://doi.org/10.1002/jgm.1035" @default.
• W1987163952 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17410614" @default.
• W1987163952 hasPublicationYear "2007" @default.
• W1987163952 type Work @default.
• W1987163952 sameAs 1987163952 @default.
• W1987163952 citedByCount "65" @default.
• W1987163952 countsByYear W19871639522012 @default.
• W1987163952 countsByYear W19871639522013 @default.
• W1987163952 countsByYear W19871639522014 @default.
• W1987163952 countsByYear W19871639522015 @default.
• W1987163952 countsByYear W19871639522016 @default.
• W1987163952 countsByYear W19871639522017 @default.
• W1987163952 countsByYear W19871639522018 @default.
• W1987163952 countsByYear W19871639522019 @default.
• W1987163952 countsByYear W19871639522021 @default.
• W1987163952 countsByYear W19871639522022 @default.
• W1987163952 countsByYear W19871639522023 @default.
• W1987163952 crossrefType "journal-article" @default.
• W1987163952 hasAuthorship W1987163952A5014958903 @default.
• W1987163952 hasAuthorship W1987163952A5040073148 @default.
• W1987163952 hasAuthorship W1987163952A5065810893 @default.
• W1987163952 hasAuthorship W1987163952A5070879563 @default.
• W1987163952 hasAuthorship W1987163952A5088605616 @default.
• W1987163952 hasAuthorship W1987163952A5091424033 @default.
• W1987163952 hasConcept C104317684 @default.
• W1987163952 hasConcept C12554922 @default.
• W1987163952 hasConcept C1491633281 @default.
• W1987163952 hasConcept C150194340 @default.
• W1987163952 hasConcept C153074725 @default.
• W1987163952 hasConcept C153911025 @default.
• W1987163952 hasConcept C161733203 @default.
• W1987163952 hasConcept C185592680 @default.
• W1987163952 hasConcept C2775925850 @default.
• W1987163952 hasConcept C2779553658 @default.
• W1987163952 hasConcept C28005876 @default.
• W1987163952 hasConcept C29537977 @default.
• W1987163952 hasConcept C40017333 @default.
• W1987163952 hasConcept C54009773 @default.
• W1987163952 hasConcept C55493867 @default.
• W1987163952 hasConcept C79879829 @default.
• W1987163952 hasConcept C86803240 @default.
• W1987163952 hasConcept C95444343 @default.
• W1987163952 hasConceptScore W1987163952C104317684 @default.
• W1987163952 hasConceptScore W1987163952C12554922 @default.
• W1987163952 hasConceptScore W1987163952C1491633281 @default.
• W1987163952 hasConceptScore W1987163952C150194340 @default.
• W1987163952 hasConceptScore W1987163952C153074725 @default.

• next