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• W1987166207 abstract "A nitric oxide (•NO) spin-trapping technique combined with electron paramagnetic resonance (EPR) spectroscopy has been employed to measure the in vivo production of •NO in lipopolysaccharide (LPS)-treated mice. The in vivo spin-trapping of •NO was performed by injecting into mice a metal—chelator complex, consisting of N-methyl-d-glucamine dithiocarbamate (MGD) and reduced iron (Fe2+), that binds to •NO and forms a stable, water-soluble [(MGD)2-Fe2+-NO] complex, and by monitoring continuously the in vivo formation of the latter complex using an S-band EPR spectrometer. At 6 h after intravenous injection of LPS, a three-line EPR spectrum of the [(MGD)2-Fe2+-NO] complex, was observed in the blood circulation of the mouse tail; the [(MGD)2-Fe2+] complex was injected subcutaneously 2 h before EPR measurement. No signal was detected in control groups. Administration of N G-monomethyl-l-arginine, an •NO synthase inhibitor, caused a marked reduction in the in vivo EPR signal of the [(MGD)2-Fe2+-NO] complex, suggesting that the •NO detected is synthesized via the arginine-nitric oxide synthase pathway. The results presented here demonstrated, for the first time, the in vivo real time measurement of •NO in the blood circulation of conscious, LPS-treated animals." @default.
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• W1987166207 date "1994-05-30" @default.
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• W1987166207 title "Spin trapping of nitric oxide produced in vivo in septic-shock mice" @default.
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• W1987166207 doi "https://doi.org/10.1016/0014-5793(94)00422-6" @default.
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