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- W1987166857 abstract "An active recombinant glycoprotein hormone, porcine follicle-stimulating hormone (recFSH), has been produced for the first time in the methylotrophic yeast,Pichia pastoris.The yield of secreted recFSH (10 mg/l) was the highest ever reached. RecFSH displayed an apparent molecular mass of 41 kDa by SDS-PAGE and was found to bear only N-linked carbohydrates of the high-mannose type. Itsin vitrobinding and cell-stimulating activities were identical to those of pituitary porcine FSH. The large availability and the noncharged N-glycans of FSHrec should render it highly valuable for structural studies." @default.
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- W1987166857 date "1998-04-01" @default.
- W1987166857 modified "2023-10-18" @default.
- W1987166857 title "High-Level Secretion of Biologically Active Recombinant Porcine Follicle-Stimulating Hormone by the Methylotrophic YeastPichia pastoris" @default.
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- W1987166857 doi "https://doi.org/10.1006/bbrc.1998.8532" @default.
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