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• W1987169898 abstract "Proton nuclear magnetic resonance (1H NMR) spectroscopy for detection of biochemical changes in biological samples is a successful technique. However, the achieved NMR resolution is not sufficiently high when the analysis is performed with intact cells. To improve spectral resolution, high resolution magic angle spinning (HR-MAS) is used and the broad signals are separated by a T2 filter based on the CPMG pulse sequence. Additionally, HR-MAS experiments with a T2 filter are preceded by a water suppression procedure. The goal of this work is to demonstrate that the experimental procedures of water suppression and T2 or diffusing filters are unnecessary steps when the filter diagonalization method (FDM) is used to process the time domain HR-MAS signals. Manipulation of the FDM results, represented as a tabular list of peak positions, widths, amplitudes and phases, allows the removal of water signals without the disturbing overlapping or nearby signals. Additionally, the FDM can also be used for phase correction and noise suppression, and to discriminate between sharp and broad lines. Results demonstrate the applicability of the FDM post-acquisition processing to obtain high quality HR-MAS spectra of heterogeneous biological materials." @default.
• W1987169898 created "2016-06-24" @default.
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• W1987169898 date "2012-01-01" @default.
• W1987169898 modified "2023-09-24" @default.
• W1987169898 title "Processing of high resolution magic angle spinning spectra of breast cancer cells by the filter diagonalization method" @default.
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• W1987169898 doi "https://doi.org/10.1039/c2an35451a" @default.
• W1987169898 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22896835" @default.
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