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- W1987201412 abstract "Four new features have been included in a phage-λ-replicon-based cosmid vector, Lorist6. A ScaI restriction site allows insertion of blunt-ended DNA, partially digested with AluI or any other restriction enzyme which creates blunt ends after cleavage. NotI and BamHI sites enable construction of libraries using double digestion with NotI + Sau3AI. The promoter of the neo gene has been substituted by a promoter derived from the tet gene of pBR322, increasing expression of the resistance gene product. A transcriptional terminator has been placed downstream from the neo gene to prevent transcription into insert DNAs." @default.
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- W1987201412 date "1987-01-01" @default.
- W1987201412 modified "2023-10-18" @default.
- W1987201412 title "Lorist6, a cosmid vector with BamHI, NotI, ScaI and Hind III cloning sites and altered neomycin phosphotransferase gene expression" @default.
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- W1987201412 doi "https://doi.org/10.1016/0378-1119(87)90017-5" @default.
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