Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987206590> ?p ?o ?g. }
- W1987206590 endingPage "1912" @default.
- W1987206590 startingPage "1901" @default.
- W1987206590 abstract "We have developed an in vitro endothelial cell (EC)-smooth muscle cell (SMC) coculture platform that can mimic either the healthy or diseased state of blood vessels. Transforming growth factor-beta1 (TGF-beta1) and heparin were introduced to the SMC cultures to upregulate the SMC differentiation markers, alpha-smooth muscle actin (alpha-SMA) and calponin (homotypic model). Interestingly, seeding of near-confluent concentrations of ECs on the SMCs (heterotypic model) induced higher levels of alpha-SMA and calponin expression in the SMC cultures than did the addition of heparin and TGF-beta1 alone. The expression levels increased further on pretreating the SMCs with TGF-beta1 and heparin before adding a near-confluent monolayer of ECs. In contrast, seeding of sparse concentrations of ECs forced the SMCs into a more hyperplastic state as determined by alpha-SMA and calponin expression. This study highlights the importance of both soluble factors and EC seeding densities when considering culture conditions; in vivo SMCs are in close proximity with and interact with a monolayer of ECs. Our study suggests that this architecture is important for healthy vascular tissue function. In addition, it shows that disruption of this architecture can be used to mimic diseased states. As the EC-SMC coculture model can mimic either a diseased or a healthy blood vessel it may be useful as a test bed for evaluating cardiovascular therapeutics." @default.
- W1987206590 created "2016-06-24" @default.
- W1987206590 creator A5055585728 @default.
- W1987206590 creator A5056562604 @default.
- W1987206590 creator A5085637012 @default.
- W1987206590 date "2010-06-01" @default.
- W1987206590 modified "2023-09-27" @default.
- W1987206590 title "Scaffold-Free <i>In Vitro</i> Arterial Mimetics: The Importance of Smooth Muscle–Endothelium Contact" @default.
- W1987206590 cites W1574424832 @default.
- W1987206590 cites W1748311474 @default.
- W1987206590 cites W1764824114 @default.
- W1987206590 cites W1912592877 @default.
- W1987206590 cites W1963711373 @default.
- W1987206590 cites W1963782714 @default.
- W1987206590 cites W1971155172 @default.
- W1987206590 cites W1972872816 @default.
- W1987206590 cites W1973018387 @default.
- W1987206590 cites W1974483946 @default.
- W1987206590 cites W1975349241 @default.
- W1987206590 cites W1977383724 @default.
- W1987206590 cites W1977798903 @default.
- W1987206590 cites W1980848131 @default.
- W1987206590 cites W1982954591 @default.
- W1987206590 cites W1985186457 @default.
- W1987206590 cites W1992546194 @default.
- W1987206590 cites W1996018144 @default.
- W1987206590 cites W1996910230 @default.
- W1987206590 cites W1997447547 @default.
- W1987206590 cites W2002204510 @default.
- W1987206590 cites W2008195383 @default.
- W1987206590 cites W2008827433 @default.
- W1987206590 cites W2013462019 @default.
- W1987206590 cites W2013634832 @default.
- W1987206590 cites W2016723544 @default.
- W1987206590 cites W2020580233 @default.
- W1987206590 cites W2021218223 @default.
- W1987206590 cites W2024045849 @default.
- W1987206590 cites W2027135709 @default.
- W1987206590 cites W2028534260 @default.
- W1987206590 cites W2030223978 @default.
- W1987206590 cites W2031128166 @default.
- W1987206590 cites W2038939848 @default.
- W1987206590 cites W2039153769 @default.
- W1987206590 cites W2039420330 @default.
- W1987206590 cites W2048908597 @default.
- W1987206590 cites W2049865748 @default.
- W1987206590 cites W2052058991 @default.
- W1987206590 cites W2053704719 @default.
- W1987206590 cites W2053716402 @default.
- W1987206590 cites W2060952558 @default.
- W1987206590 cites W2067870542 @default.
- W1987206590 cites W2068385607 @default.
- W1987206590 cites W2070062282 @default.
- W1987206590 cites W2076597032 @default.
- W1987206590 cites W2084277357 @default.
- W1987206590 cites W2089881457 @default.
- W1987206590 cites W2094891357 @default.
- W1987206590 cites W2098036955 @default.
- W1987206590 cites W2105012708 @default.
- W1987206590 cites W2107781166 @default.
- W1987206590 cites W2112711017 @default.
- W1987206590 cites W2113248382 @default.
- W1987206590 cites W2123231252 @default.
- W1987206590 cites W2130846642 @default.
- W1987206590 cites W2133204161 @default.
- W1987206590 cites W2137570167 @default.
- W1987206590 cites W2151347388 @default.
- W1987206590 cites W2156740811 @default.
- W1987206590 cites W2170202796 @default.
- W1987206590 cites W2171284332 @default.
- W1987206590 cites W2599095084 @default.
- W1987206590 cites W2796190 @default.
- W1987206590 doi "https://doi.org/10.1089/ten.tea.2009.0271" @default.
- W1987206590 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2949266" @default.
- W1987206590 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20088699" @default.
- W1987206590 hasPublicationYear "2010" @default.
- W1987206590 type Work @default.
- W1987206590 sameAs 1987206590 @default.
- W1987206590 citedByCount "25" @default.
- W1987206590 countsByYear W19872065902012 @default.
- W1987206590 countsByYear W19872065902013 @default.
- W1987206590 countsByYear W19872065902014 @default.
- W1987206590 countsByYear W19872065902015 @default.
- W1987206590 countsByYear W19872065902016 @default.
- W1987206590 countsByYear W19872065902020 @default.
- W1987206590 countsByYear W19872065902021 @default.
- W1987206590 countsByYear W19872065902022 @default.
- W1987206590 crossrefType "journal-article" @default.
- W1987206590 hasAuthorship W1987206590A5055585728 @default.
- W1987206590 hasAuthorship W1987206590A5056562604 @default.
- W1987206590 hasAuthorship W1987206590A5085637012 @default.
- W1987206590 hasBestOaLocation W19872065902 @default.
- W1987206590 hasConcept C114614502 @default.
- W1987206590 hasConcept C118131993 @default.
- W1987206590 hasConcept C125705527 @default.
- W1987206590 hasConcept C134018914 @default.
- W1987206590 hasConcept C136229726 @default.
- W1987206590 hasConcept C150903083 @default.