FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987207856> ?p ?o ?g. }

• W1987207856 endingPage "3059" @default.
• W1987207856 startingPage "3047" @default.
• W1987207856 abstract "Growing bacterial cells forming division septa have sites near the septa that are sensitive to EDTA shock. Cells treated with EDTA incorporate proteins and other molecules from the surrounding medium, probably via vesiclelike lesions at the septa that are induced by EDTA. The amount of protein taken up is proportional to the protein concentration in the permeabilization medium. Incorporated molecules equilibrate throughout the cytoplasm, and those with affinity for DNA bind to the nucleoid. Conditions that promote the viability of permeabilized cells and help to avoid otherwise irreversible effects of EDTA are defined. Procedures for selecting cells that have incorporated protein and for studying the distribution of the protein and its effects in growing-dividing cells are described. The procedure may have several applications to molecular and cellular biology; however, we describe here the localization in living cells of the histonelike protein HU. Fluorescence microscopy of cells containing different amounts of fluorescein-labeled HU (varied from approximately 10(3) to 10(5) molecules per cell) showed that the HU concentrates in the nucleoid and is uniformly distributed throughout this structure. Control experiments demonstrated that unlabeled interior parts of the nucleoid can be resolved when labeled proteins that do not bind DNA or enter the nucleoid are introduced into living cells. It was concluded that in vivo added HU binds primarily DNA and that there are no intrinsic restrictions on major regions of the nucleoid to which the added HU protein may bind." @default.
• W1987207856 created "2016-06-24" @default.
• W1987207856 creator A5042579669 @default.
• W1987207856 creator A5062600178 @default.
• W1987207856 date "1991-05-01" @default.
• W1987207856 modified "2023-09-26" @default.
• W1987207856 title "Introduction of proteins into living bacterial cells: distribution of labeled HU protein in Escherichia coli" @default.
• W1987207856 cites W104451405 @default.
• W1987207856 cites W1496379245 @default.
• W1987207856 cites W1535183216 @default.
• W1987207856 cites W1548338247 @default.
• W1987207856 cites W1548938191 @default.
• W1987207856 cites W1552284012 @default.
• W1987207856 cites W1564563877 @default.
• W1987207856 cites W156939059 @default.
• W1987207856 cites W1575636604 @default.
• W1987207856 cites W1577909016 @default.
• W1987207856 cites W1598526304 @default.
• W1987207856 cites W1602215303 @default.
• W1987207856 cites W1615284855 @default.
• W1987207856 cites W1695535299 @default.
• W1987207856 cites W1759607853 @default.
• W1987207856 cites W1966856085 @default.
• W1987207856 cites W1980413301 @default.
• W1987207856 cites W1983297701 @default.
• W1987207856 cites W1993045940 @default.
• W1987207856 cites W1998431315 @default.
• W1987207856 cites W2006605582 @default.
• W1987207856 cites W2009966858 @default.
• W1987207856 cites W2010598074 @default.
• W1987207856 cites W2035207357 @default.
• W1987207856 cites W2041402415 @default.
• W1987207856 cites W2042976980 @default.
• W1987207856 cites W2064030857 @default.
• W1987207856 cites W2069143195 @default.
• W1987207856 cites W2084623901 @default.
• W1987207856 cites W2102937661 @default.
• W1987207856 cites W2107541106 @default.
• W1987207856 cites W2111811278 @default.
• W1987207856 cites W2152939667 @default.
• W1987207856 cites W2398875252 @default.
• W1987207856 doi "https://doi.org/10.1128/jb.173.10.3047-3059.1991" @default.
• W1987207856 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/207897" @default.
• W1987207856 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/1902461" @default.
• W1987207856 hasPublicationYear "1991" @default.
• W1987207856 type Work @default.
• W1987207856 sameAs 1987207856 @default.
• W1987207856 citedByCount "43" @default.
• W1987207856 countsByYear W19872078562012 @default.
• W1987207856 countsByYear W19872078562014 @default.
• W1987207856 countsByYear W19872078562019 @default.
• W1987207856 crossrefType "journal-article" @default.
• W1987207856 hasAuthorship W1987207856A5042579669 @default.
• W1987207856 hasAuthorship W1987207856A5062600178 @default.
• W1987207856 hasBestOaLocation W19872078562 @default.
• W1987207856 hasConcept C104317684 @default.
• W1987207856 hasConcept C121332964 @default.
• W1987207856 hasConcept C12554922 @default.
• W1987207856 hasConcept C1491633281 @default.
• W1987207856 hasConcept C153911025 @default.
• W1987207856 hasConcept C169274487 @default.
• W1987207856 hasConcept C190062978 @default.
• W1987207856 hasConcept C53642103 @default.
• W1987207856 hasConcept C547475151 @default.
• W1987207856 hasConcept C552990157 @default.
• W1987207856 hasConcept C55493867 @default.
• W1987207856 hasConcept C62520636 @default.
• W1987207856 hasConcept C85813293 @default.
• W1987207856 hasConcept C86339819 @default.
• W1987207856 hasConcept C86803240 @default.
• W1987207856 hasConcept C91881484 @default.
• W1987207856 hasConcept C94966510 @default.
• W1987207856 hasConcept C95444343 @default.
• W1987207856 hasConceptScore W1987207856C104317684 @default.
• W1987207856 hasConceptScore W1987207856C121332964 @default.
• W1987207856 hasConceptScore W1987207856C12554922 @default.
• W1987207856 hasConceptScore W1987207856C1491633281 @default.
• W1987207856 hasConceptScore W1987207856C153911025 @default.
• W1987207856 hasConceptScore W1987207856C169274487 @default.
• W1987207856 hasConceptScore W1987207856C190062978 @default.
• W1987207856 hasConceptScore W1987207856C53642103 @default.
• W1987207856 hasConceptScore W1987207856C547475151 @default.
• W1987207856 hasConceptScore W1987207856C552990157 @default.
• W1987207856 hasConceptScore W1987207856C55493867 @default.
• W1987207856 hasConceptScore W1987207856C62520636 @default.
• W1987207856 hasConceptScore W1987207856C85813293 @default.
• W1987207856 hasConceptScore W1987207856C86339819 @default.
• W1987207856 hasConceptScore W1987207856C86803240 @default.
• W1987207856 hasConceptScore W1987207856C91881484 @default.
• W1987207856 hasConceptScore W1987207856C94966510 @default.
• W1987207856 hasConceptScore W1987207856C95444343 @default.
• W1987207856 hasIssue "10" @default.
• W1987207856 hasLocation W19872078561 @default.
• W1987207856 hasLocation W19872078562 @default.
• W1987207856 hasLocation W19872078563 @default.
• W1987207856 hasLocation W19872078564 @default.
• W1987207856 hasOpenAccess W1987207856 @default.
• W1987207856 hasPrimaryLocation W19872078561 @default.

• next