Matches in SemOpenAlex for { <https://semopenalex.org/work/W198721574> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W198721574 endingPage "123" @default.
- W198721574 startingPage "107" @default.
- W198721574 abstract "RNA interference (RNAi) represents the most frequently utilized technique to analyze proteins by loss of function assays. Protein synthesis is impaired by sequence-specific degradation of mRNA, which is triggered by short (19–28 nt) silencing RNAs (siRNA). Efficient gene silencing using RNAi has been demonstrated in numerous cell lines and primary cultured cells. Incorporation of siRNA into terminally differentiated mammalian cells, such as adult cardiac myocytes is limited by their resistance to standard transfection protocols. Viral delivery of short-hairpin RNA (shRNA) overcomes these limitations and allows efficient gene silencing in these cells. This chapter describes the generation and characterization of recombinant siRNA-encoding adenoviruses and their application to adult cardiac myocytes, which represent a standard experimental model in research related to cardiac physiology and pathophysiology. Feasibility of this approach is demonstrated by effective ablation (>80%) of both, a transgene encoding for eGFP and the endogenous muscarinic M2 acetylcholine receptor." @default.
- W198721574 created "2016-06-24" @default.
- W198721574 creator A5014267602 @default.
- W198721574 creator A5034574485 @default.
- W198721574 creator A5047291023 @default.
- W198721574 creator A5067150916 @default.
- W198721574 creator A5074682022 @default.
- W198721574 date "2009-01-01" @default.
- W198721574 modified "2023-10-17" @default.
- W198721574 title "Adenovirus-Mediated Delivery of Short Hairpin RNA (shRNA) Mediates Efficient Gene Silencing in Terminally Differentiated Cardiac Myocytes" @default.
- W198721574 cites W1611419331 @default.
- W198721574 cites W1980912932 @default.
- W198721574 cites W1999846165 @default.
- W198721574 cites W2048830597 @default.
- W198721574 cites W2079615819 @default.
- W198721574 cites W2091225710 @default.
- W198721574 cites W2097131232 @default.
- W198721574 cites W2150505696 @default.
- W198721574 cites W2153747430 @default.
- W198721574 cites W2158627784 @default.
- W198721574 cites W2170781554 @default.
- W198721574 cites W4253422011 @default.
- W198721574 doi "https://doi.org/10.1007/978-1-59745-559-6_7" @default.
- W198721574 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19378115" @default.
- W198721574 hasPublicationYear "2009" @default.
- W198721574 type Work @default.
- W198721574 sameAs 198721574 @default.
- W198721574 citedByCount "11" @default.
- W198721574 countsByYear W1987215742013 @default.
- W198721574 countsByYear W1987215742014 @default.
- W198721574 countsByYear W1987215742015 @default.
- W198721574 countsByYear W1987215742021 @default.
- W198721574 crossrefType "book-chapter" @default.
- W198721574 hasAuthorship W198721574A5014267602 @default.
- W198721574 hasAuthorship W198721574A5034574485 @default.
- W198721574 hasAuthorship W198721574A5047291023 @default.
- W198721574 hasAuthorship W198721574A5067150916 @default.
- W198721574 hasAuthorship W198721574A5074682022 @default.
- W198721574 hasConcept C104317684 @default.
- W198721574 hasConcept C119056186 @default.
- W198721574 hasConcept C153911025 @default.
- W198721574 hasConcept C155330019 @default.
- W198721574 hasConcept C166703698 @default.
- W198721574 hasConcept C190232843 @default.
- W198721574 hasConcept C22615655 @default.
- W198721574 hasConcept C54009773 @default.
- W198721574 hasConcept C54355233 @default.
- W198721574 hasConcept C67705224 @default.
- W198721574 hasConcept C86803240 @default.
- W198721574 hasConcept C95444343 @default.
- W198721574 hasConceptScore W198721574C104317684 @default.
- W198721574 hasConceptScore W198721574C119056186 @default.
- W198721574 hasConceptScore W198721574C153911025 @default.
- W198721574 hasConceptScore W198721574C155330019 @default.
- W198721574 hasConceptScore W198721574C166703698 @default.
- W198721574 hasConceptScore W198721574C190232843 @default.
- W198721574 hasConceptScore W198721574C22615655 @default.
- W198721574 hasConceptScore W198721574C54009773 @default.
- W198721574 hasConceptScore W198721574C54355233 @default.
- W198721574 hasConceptScore W198721574C67705224 @default.
- W198721574 hasConceptScore W198721574C86803240 @default.
- W198721574 hasConceptScore W198721574C95444343 @default.
- W198721574 hasLocation W1987215741 @default.
- W198721574 hasLocation W1987215742 @default.
- W198721574 hasOpenAccess W198721574 @default.
- W198721574 hasPrimaryLocation W1987215741 @default.
- W198721574 hasRelatedWork W1981390890 @default.
- W198721574 hasRelatedWork W1993840407 @default.
- W198721574 hasRelatedWork W2003755164 @default.
- W198721574 hasRelatedWork W2014913386 @default.
- W198721574 hasRelatedWork W2042993634 @default.
- W198721574 hasRelatedWork W2083004731 @default.
- W198721574 hasRelatedWork W2335561318 @default.
- W198721574 hasRelatedWork W2373539659 @default.
- W198721574 hasRelatedWork W2387605231 @default.
- W198721574 hasRelatedWork W2421873547 @default.
- W198721574 isParatext "false" @default.
- W198721574 isRetracted "false" @default.
- W198721574 magId "198721574" @default.
- W198721574 workType "book-chapter" @default.