Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987281023> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W1987281023 endingPage "42" @default.
- W1987281023 startingPage "37" @default.
- W1987281023 abstract "Plasma amino acid concentrations fall during insulin infusion. Amino acid concentrations can be maintained using an infusion of amino acids if their plasma concentration can be determined within a few minutes. We developed a spectrophometric assay which determines the total concentration of all three branched-chain amino acids in plasma within 1 min. The enzyme leucine dehydrogenase oxidatively deaminates leucine, isoleucine, and valine, with stoichiometric reduction of NAD that is measured using a spectrophotometer. The assay was developed in both a kinetic and end-point format. For the kinetic assay the buffer conditions were formulated to obtain equivalent rates with all three amino acids so that it could be used in samples containing unknown mixtures. For the end-point assay additional enzyme was added so that an end-point could be reached within 1 min. The application of the kinetic assay for “clamping” the branched-chain amino acids during hyperinsulinemic euglycemic clamps in humans is demonstrated." @default.
- W1987281023 created "2016-06-24" @default.
- W1987281023 creator A5034383966 @default.
- W1987281023 creator A5067530536 @default.
- W1987281023 creator A5083198580 @default.
- W1987281023 date "1985-11-01" @default.
- W1987281023 modified "2023-10-18" @default.
- W1987281023 title "Simple enzymatic method for plasma branched-chain amino acids" @default.
- W1987281023 cites W1480850078 @default.
- W1987281023 cites W1896604741 @default.
- W1987281023 cites W2035442120 @default.
- W1987281023 cites W4300676711 @default.
- W1987281023 doi "https://doi.org/10.1016/0009-8981(85)90136-6" @default.
- W1987281023 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4075518" @default.
- W1987281023 hasPublicationYear "1985" @default.
- W1987281023 type Work @default.
- W1987281023 sameAs 1987281023 @default.
- W1987281023 citedByCount "12" @default.
- W1987281023 crossrefType "journal-article" @default.
- W1987281023 hasAuthorship W1987281023A5034383966 @default.
- W1987281023 hasAuthorship W1987281023A5067530536 @default.
- W1987281023 hasAuthorship W1987281023A5083198580 @default.
- W1987281023 hasConcept C181199279 @default.
- W1987281023 hasConcept C185592680 @default.
- W1987281023 hasConcept C2776580952 @default.
- W1987281023 hasConcept C2777305461 @default.
- W1987281023 hasConcept C2777573094 @default.
- W1987281023 hasConcept C2779856020 @default.
- W1987281023 hasConcept C43617362 @default.
- W1987281023 hasConcept C515207424 @default.
- W1987281023 hasConcept C55493867 @default.
- W1987281023 hasConceptScore W1987281023C181199279 @default.
- W1987281023 hasConceptScore W1987281023C185592680 @default.
- W1987281023 hasConceptScore W1987281023C2776580952 @default.
- W1987281023 hasConceptScore W1987281023C2777305461 @default.
- W1987281023 hasConceptScore W1987281023C2777573094 @default.
- W1987281023 hasConceptScore W1987281023C2779856020 @default.
- W1987281023 hasConceptScore W1987281023C43617362 @default.
- W1987281023 hasConceptScore W1987281023C515207424 @default.
- W1987281023 hasConceptScore W1987281023C55493867 @default.
- W1987281023 hasIssue "1" @default.
- W1987281023 hasLocation W19872810231 @default.
- W1987281023 hasLocation W19872810232 @default.
- W1987281023 hasOpenAccess W1987281023 @default.
- W1987281023 hasPrimaryLocation W19872810231 @default.
- W1987281023 hasRelatedWork W1899415214 @default.
- W1987281023 hasRelatedWork W1975808600 @default.
- W1987281023 hasRelatedWork W1993795649 @default.
- W1987281023 hasRelatedWork W2067456796 @default.
- W1987281023 hasRelatedWork W2078519908 @default.
- W1987281023 hasRelatedWork W2079291036 @default.
- W1987281023 hasRelatedWork W2080181011 @default.
- W1987281023 hasRelatedWork W2281211411 @default.
- W1987281023 hasRelatedWork W2473103804 @default.
- W1987281023 hasRelatedWork W3131068657 @default.
- W1987281023 hasVolume "153" @default.
- W1987281023 isParatext "false" @default.
- W1987281023 isRetracted "false" @default.
- W1987281023 magId "1987281023" @default.
- W1987281023 workType "article" @default.