FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W1987282023> ?p ?o ?g. }

• W1987282023 endingPage "e25857" @default.
• W1987282023 startingPage "e25857" @default.
• W1987282023 abstract "Regulatory T cells (Tregs) were identified several years ago and are key in controlling autoimmune diseases and limiting immune responses to foreign antigens, including alloantigens. In vivo imaging techniques including intravital microscopy as well as whole body imaging using bioluminescence probes have contributed to the understanding of in vivo Treg function, their mechanisms of action and target cells. Imaging of the human sodium/iodide symporter via Single Photon Emission Computed Tomography (SPECT) has been used to image various cell types in vivo. It has several advantages over the aforementioned imaging techniques including high sensitivity, it allows non-invasive whole body studies of viable cell migration and localisation of cells over time and lastly it may offer the possibility to be translated to the clinic. This study addresses whether SPECT/CT imaging can be used to visualise the migratory pattern of Tregs in vivo. Treg lines derived from CD4+CD25+FoxP3+ cells were retrovirally transduced with a construct encoding for the human Sodium Iodide Symporter (NIS) and the fluorescent protein mCherry and stimulated with autologous DCs. NIS expressing self-specific Tregs were specifically radiolabelled in vitro with Technetium-99m pertechnetate (99mTcO4−) and exposure of these cells to radioactivity did not affect cell viability, phenotype or function. In addition adoptively transferred Treg-NIS cells were imaged in vivo in C57BL/6 (BL/6) mice by SPECT/CT using 99mTcO4−. After 24 hours NIS expressing Tregs were observed in the spleen and their localisation was further confirmed by organ biodistribution studies and flow cytometry analysis. The data presented here suggests that SPECT/CT imaging can be utilised in preclinical imaging studies of adoptively transferred Tregs without affecting Treg function and viability thereby allowing longitudinal studies within disease models." @default.
• W1987282023 created "2016-06-24" @default.
• W1987282023 creator A5001550874 @default.
• W1987282023 creator A5010690912 @default.
• W1987282023 creator A5017494077 @default.
• W1987282023 creator A5017814506 @default.
• W1987282023 creator A5019533207 @default.
• W1987282023 creator A5021192229 @default.
• W1987282023 creator A5035326990 @default.
• W1987282023 creator A5041610357 @default.
• W1987282023 creator A5049873829 @default.
• W1987282023 creator A5067102040 @default.
• W1987282023 creator A5086830790 @default.
• W1987282023 creator A5090174053 @default.
• W1987282023 date "2011-10-17" @default.
• W1987282023 modified "2023-09-26" @default.
• W1987282023 title "In Vivo SPECT Reporter Gene Imaging of Regulatory T Cells" @default.
• W1987282023 cites W1520290955 @default.
• W1987282023 cites W1986646780 @default.
• W1987282023 cites W1988785107 @default.
• W1987282023 cites W2001256439 @default.
• W1987282023 cites W2016026517 @default.
• W1987282023 cites W2018715802 @default.
• W1987282023 cites W2027488189 @default.
• W1987282023 cites W2034337172 @default.
• W1987282023 cites W2034340892 @default.
• W1987282023 cites W2038446250 @default.
• W1987282023 cites W2055603849 @default.
• W1987282023 cites W2065003195 @default.
• W1987282023 cites W2068392572 @default.
• W1987282023 cites W2069832552 @default.
• W1987282023 cites W2072853625 @default.
• W1987282023 cites W2080092936 @default.
• W1987282023 cites W2084416533 @default.
• W1987282023 cites W2091518998 @default.
• W1987282023 cites W2094203088 @default.
• W1987282023 cites W2101452381 @default.
• W1987282023 cites W2105163419 @default.
• W1987282023 cites W2110537907 @default.
• W1987282023 cites W2115734610 @default.
• W1987282023 cites W2119507443 @default.
• W1987282023 cites W2151428570 @default.
• W1987282023 cites W2153389047 @default.
• W1987282023 cites W2155521069 @default.
• W1987282023 cites W2158715209 @default.
• W1987282023 cites W2166676682 @default.
• W1987282023 cites W2329507162 @default.
• W1987282023 cites W2351941869 @default.
• W1987282023 doi "https://doi.org/10.1371/journal.pone.0025857" @default.
• W1987282023 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3197183" @default.
• W1987282023 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22043296" @default.
• W1987282023 hasPublicationYear "2011" @default.
• W1987282023 type Work @default.
• W1987282023 sameAs 1987282023 @default.
• W1987282023 citedByCount "40" @default.
• W1987282023 countsByYear W19872820232012 @default.
• W1987282023 countsByYear W19872820232013 @default.
• W1987282023 countsByYear W19872820232014 @default.
• W1987282023 countsByYear W19872820232015 @default.
• W1987282023 countsByYear W19872820232016 @default.
• W1987282023 countsByYear W19872820232017 @default.
• W1987282023 countsByYear W19872820232018 @default.
• W1987282023 countsByYear W19872820232019 @default.
• W1987282023 countsByYear W19872820232020 @default.
• W1987282023 countsByYear W19872820232021 @default.
• W1987282023 countsByYear W19872820232022 @default.
• W1987282023 countsByYear W19872820232023 @default.
• W1987282023 crossrefType "journal-article" @default.
• W1987282023 hasAuthorship W1987282023A5001550874 @default.
• W1987282023 hasAuthorship W1987282023A5010690912 @default.
• W1987282023 hasAuthorship W1987282023A5017494077 @default.
• W1987282023 hasAuthorship W1987282023A5017814506 @default.
• W1987282023 hasAuthorship W1987282023A5019533207 @default.
• W1987282023 hasAuthorship W1987282023A5021192229 @default.
• W1987282023 hasAuthorship W1987282023A5035326990 @default.
• W1987282023 hasAuthorship W1987282023A5041610357 @default.
• W1987282023 hasAuthorship W1987282023A5049873829 @default.
• W1987282023 hasAuthorship W1987282023A5067102040 @default.
• W1987282023 hasAuthorship W1987282023A5086830790 @default.
• W1987282023 hasAuthorship W1987282023A5090174053 @default.
• W1987282023 hasBestOaLocation W19872820231 @default.
• W1987282023 hasConcept C104317684 @default.
• W1987282023 hasConcept C111335760 @default.
• W1987282023 hasConcept C120405084 @default.
• W1987282023 hasConcept C136339569 @default.
• W1987282023 hasConcept C149011108 @default.
• W1987282023 hasConcept C150903083 @default.
• W1987282023 hasConcept C161997846 @default.
• W1987282023 hasConcept C203014093 @default.
• W1987282023 hasConcept C207001950 @default.
• W1987282023 hasConcept C2777807558 @default.
• W1987282023 hasConcept C2779727006 @default.
• W1987282023 hasConcept C2780616701 @default.
• W1987282023 hasConcept C29331040 @default.
• W1987282023 hasConcept C2984398910 @default.
• W1987282023 hasConcept C2989005 @default.
• W1987282023 hasConcept C502942594 @default.
• W1987282023 hasConcept C54009773 @default.

• next