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- W1987293640 abstract "Unmarked gene modifications are desirable for various genetic analyses; however, they have been difficult to construct without selectable markers. We describe here a new genetic method for constructing unmarked mutants in Streptococcus mutans. The desired mutant allele is first constructed and introduced into the strain by cotransformation with pGhost4, a thermosensitive plasmid that replicates in several low G+C Gram positive bacteria. With this method, we have modified two different loci with high frequency by insertion or deletion in S. mutans. Because pGhost4 contains a broad host range thermosensitive replicon, this method can be applied to any transformable low G+C Gram positive bacteria, including oral streptococci." @default.
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- W1987293640 date "2007-04-01" @default.
- W1987293640 modified "2023-09-25" @default.
- W1987293640 title "Unmarked gene modification in <i>Streptococcus mutans</i> by a cotransformation strategy with a thermosensitive plasmid" @default.
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- W1987293640 doi "https://doi.org/10.2144/000112414" @default.
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